Role of fatty acid elongase Elovl6 in the sucrose preference formation

我们研究了elov16缺乏对小鼠食物摄入和食物偏好的影响。本研究使用了三种类型的日粮，野生型（WT）和elov16基因敲除（KO）小鼠同时使用一种或两种日粮。在实验1中，小鼠被喂食标准食物、高脂肪或高糖食物。实验2，WT和Elovl6KO小鼠分别饲喂饲粮、鼠粮和HF、HS和HF、鼠粮和HS三对中的一种。在单独饲养的小鼠中依次监测食物摄入量。在实验1中，Elovl6KO小鼠比WT小鼠对HS饮食的摄取量增加。相比之下，WT和Elovl6KO小鼠的标准饲料和HF饮食摄入量相似。在实验2中，与WT小鼠相比，Elovl6KO小鼠对HS饮食的偏好大于对chow饮食的偏好，但对chow饮食和HF饮食或HS对HF饮食的偏好相同。在鼠粮和HS日粮的情况下，WT和Elovl6KO小鼠的食物总摄取量和卡路里摄取量（HS和HS日粮的总和）相似，因为Elovl6KO小鼠在所有时间点的鼠粮摄取量都有所减少。Elovl6KO小鼠对HS饮食的偏好明显增强。这些结果表明，Elovl6可能在控制蔗糖适口性等享乐性饮食中发挥特殊而独特的作用，但对体内平衡饮食没有影响。

We investigated the effects of Elovl6 deficiency on the food intake and food preference in mice. Three types of diets were used in this study, with one or two diets simultaneously available to wild-type (WT) and Elovl6 knockout (KO) mice. In Experiment 1, mice were fed a standard chow, high fat, or high sucrose diet. In Experiment 2, WT and Elovl6KO mice were fed one of three pairs of diet, chow and HF, HS and HF, or chow and HS. Food intake was sequentially monitored in individually caged mice. In Experiment 1, Elovl6KO mice showed an enhanced ingestion of HS diet than WT mice. In contrast, the standard chow and HF diet intake were similar between WT and Elovl6KO mice. In Experiment 2, as compared with WT mice, Elovl6KO mice showed a greater preference for HS diet than a chow diet, but exhibited an equivalent preference for chow versus HF diet, or HS versus HF diet. With chow and HS diets, the total amount of food and calorie intake, which are the sum of HS and chow diet, were similar between WT and Elovl6 KO mice because chow diet consumption was decreased at all time point in Elovl6KO mice. Elovl6KO mice demonstrated a clear enhanced preference for HS diet. These results indicate that Elovl6 may play a specific and unique role in the control of hedonic eating such as sucrose palatability, but have no effect on homeostatic eating.