TRPV4, a Ca ion channel plays pivotal role in chondrogenic mechanotransduction in ATDC5

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It is well known that chondrocyte metabolic activity is partly regulated by physical factors.However, the specific cellular mechanosensor responsible for perception and transduction has not been fully identified in chondrocytes. It has already reported that TRPV4 activation can contribute to the process of chondrogenesis via SOX9 pathway.In this study we demonstrated that TRPV4 works as a mechanoreceptor in response to mechanical stress in chondrocytes, and to determine the intracellular mechanisms leading to the chondorogensis. Our conclusions are as follow, Mechanical stress promoted chondorogensis in ATDC5 cells with increasing mRNA expression of SOX9. Inhibition of TRPV4 by chmical and siRNA suppressed chondrogenesis effect of mechanical stress in ATDC5 cells. Ca influx by mechanical stress was suppressed by RR supplementation and TRPV4 siRNA transfection.