The application of osteoblast-like cells and platelet rich plasma gels transplantation for accelerating the bone regeneration in the distraction osteogenesis.

成骨细胞样细胞和富血小板血浆凝胶移植在牵引成骨中加速骨再生的应用。

• 批准号: 14370461
• 负责人: ISHIGURO Naoki
• 金额: $ 6.72万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14370461/
• 关键词: distraction osteogenesis; tissue engineering; bone regeneration; osteoblast; mesenchymal stem cells; clinical application; 組識工学

Distraction osteogenesis has been successfully used for limb lengthening in patients with limb length discrepancy, severe short stature due to skeletal dysplasias. Although this treatment method is biological and has many advantages for these pathological conditions, the periods of external fixation and bone maturation is long which results in higher rates of complications such as pin track infection, joint contractures, pin loosening, delayed consolidations and fractures. The distraction osteogenesis is considered as bone regeneration models in human. So we developed rat models, and to investigate the possibility of accelerating bone formation of the distracted callus with usage of combination of cells and suitable scaffold. Bone marrow contains a population of multipotent mesenchymal stem cells(MSCs) that can be directed towards the osteogenic lineage in vitro if cultured in the presence of dexamethasone, β-glycerophosphate and ascorbic acid. Using the rat limb lengthening model, we … More revealed that transplantation of marrow derived osteoblast-like cells with collagen gel into the distracted callus promoted new bone formation and shortened the consolidation period. Also the hrFGF-2 and hrBMP-2 could stimulate the matrix synthesis and proliferation of this osteoblast-like cells derived from MSCs. The osteogenic potential of rat MSCs were reduced with passaging. In the P3 cells, osteogenic potential was severely suppressed. After acquiring these insights, we planed the clinical application of osteoblast-like cell transplantation. We started the clinical research of limb lengthening with osteoblast-like cells and plate rich plasma gels for achondroplasia and hypochondoroplasia patients under the supervision of institutional reviewer boards in Nagoya university hospital. Although the results were preliminary, transplantation of osteoblast-like cells and PRP could shorten the treatment period by acceleration of bone regeneration during distraction osteogenesis. It was clearly demantrated that eatablishiment of the culture conditions permitting the rapid expansion of MSCs is required for improvement of clinical results. Less

牵张成骨已成功用于肢体长度不等、因骨骼发育不良导致的严重身材矮小的患者的肢体延长。虽然这种治疗方法是生物学的，并且对于这些病理条件具有许多优点，但是外固定和骨成熟的时间较长，这导致并发症的发生率较高，例如针道感染、关节挛缩、针松动、延迟巩固和骨折。牵张成骨被认为是人类骨再生的模型。因此，我们建立了大鼠牵张骨痂成骨模型，探讨细胞与合适的支架材料结合促进牵张骨痂成骨的可能性。骨髓含有多能间充质干细胞（MSC）群体，如果在地塞米松、β-甘油磷酸盐和抗坏血酸的存在下培养，所述多能间充质干细胞（MSC）群体可以在体外定向成骨谱系。利用大鼠肢体延长模型， ...更多信息 结果显示，骨髓源性成骨样细胞与胶原凝胶一起移植到牵张的骨痂中，促进了新骨形成，缩短了巩固期。hrFGF-2和hrBMP-2可促进MSCs来源的成骨样细胞的基质合成和增殖。随着传代次数的增加，大鼠MSCs的成骨潜能降低。在P3细胞中，成骨潜能被严重抑制。在此基础上，我们对成骨样细胞移植的临床应用进行了规划。我们在名古屋大学医院的机构审查委员会的监督下，开始了用成骨细胞样细胞和富含板的血浆凝胶对软骨发育不全和软骨发育不良患者进行肢体延长的临床研究。虽然结果是初步的，但成骨细胞样细胞和PRP的移植可以通过加速牵张成骨过程中的骨再生来缩短治疗时间。明确要求建立允许MSC快速扩增的培养条件以改善临床结果。少

项目成果

Hideki Warashina, Shinji Sakano, Shinji Kitamura, Kenichi Yamauchi, Jin Yamaguchi, Naoki Ishiguro: "Biological reaction to alumina, zirconia. titanium and polyethylene particles implanted onto murinr calvaria"Biomaterials. 24. 3655-3661 (2003)

Hideki Warashina、Shinji Sakano、Shinji Kitamura、Kenichi Yamauchi、Jin Yamaguchi、Naoki Ishiguro：“植入小鼠颅骨上的氧化铝、氧化锆、钛和聚乙烯颗粒的生物反应”生物材料。

Distraction Osteogenesis Enhaaced by Osteoblastlike Cells and Collagen Gel

成骨细胞和胶原凝胶促进牵引成骨

• 发表时间: 2002
• 期刊: Clin Orthop 399
• 作者: Yuji Takamine;Hiroki Tsuchiya;Takahiko Kitakoji;Kazuhiro Kurita;Yoshihiro Ono;Y.Ohshima;Hiroshi Kitoh;Naoki Ishiguro et al.
• 通讯作者: Naoki Ishiguro et al.

Uni-axial cyclic stretch induces the activation of transcription factor nuclear factor κ B in human fibroblast cells

单轴循环拉伸诱导人成纤维细胞转录因子核因子κB的激活

• 发表时间: 2002
• 期刊: FASEB Jornal 16
• 作者: Hidefumi Inoh;Naoki Ishiguro;Shin-Ichi Sawazaki;Hideki Amma et al.
• 通讯作者: Hideki Amma et al.

Wannarat Yingsung, L Zhuo, M Morgelin, M Yoneda, D Kida, H Watabe, Naoki Ishiguro, et al.: "Molecular heterogeneity of the SHAP-hyaluronan complex. Isolation and characterization of the complex in synovial fluid from patients with rheumatoid arthritis"J B

Wannarat Yingsung、L Zhuo、M Morgelin、M Yoneda、D Kida、H Watabe、Naoki Ishiguro 等人：“SHAP-透明质酸复合物的分子异质性。类风湿性关节炎患者滑液中复合物的分离和表征”

Knee-spine syndrome: correlation between sacral inclination and patellofemoral joint pain.

• DOI: 10.1007/s007760200092
• 发表时间: 2002-01-01
• 期刊: Journal of orthopaedic science : official journal of the Japanese Orthopaedic Association
• 作者: Tsuji, Taichi;Matsuyama, Yukihiro;Ishiguro, Naoki
• 通讯作者: Ishiguro, Naoki