The application of osteoblast-like cells and platelet rich plasma gels transplantation for accelerating the bone regeneration in the distraction osteogenesis.

点击翻译按钮获取中文摘要

Distraction osteogenesis has been successfully used for limb lengthening in patients with limb length discrepancy, severe short stature due to skeletal dysplasias. Although this treatment method is biological and has many advantages for these pathological conditions, the periods of external fixation and bone maturation is long which results in higher rates of complications such as pin track infection, joint contractures, pin loosening, delayed consolidations and fractures. The distraction osteogenesis is considered as bone regeneration models in human. So we developed rat models, and to investigate the possibility of accelerating bone formation of the distracted callus with usage of combination of cells and suitable scaffold. Bone marrow contains a population of multipotent mesenchymal stem cells(MSCs) that can be directed towards the osteogenic lineage in vitro if cultured in the presence of dexamethasone, β-glycerophosphate and ascorbic acid. Using the rat limb lengthening model, we … More revealed that transplantation of marrow derived osteoblast-like cells with collagen gel into the distracted callus promoted new bone formation and shortened the consolidation period. Also the hrFGF-2 and hrBMP-2 could stimulate the matrix synthesis and proliferation of this osteoblast-like cells derived from MSCs. The osteogenic potential of rat MSCs were reduced with passaging. In the P3 cells, osteogenic potential was severely suppressed. After acquiring these insights, we planed the clinical application of osteoblast-like cell transplantation. We started the clinical research of limb lengthening with osteoblast-like cells and plate rich plasma gels for achondroplasia and hypochondoroplasia patients under the supervision of institutional reviewer boards in Nagoya university hospital. Although the results were preliminary, transplantation of osteoblast-like cells and PRP could shorten the treatment period by acceleration of bone regeneration during distraction osteogenesis. It was clearly demantrated that eatablishiment of the culture conditions permitting the rapid expansion of MSCs is required for improvement of clinical results. Less