The development of tissue engineering procedure for osteogensesis -investigation using plate rich plasma and mesenchymal stem cell-

牵引成骨(DO)已成为治疗四肢不等长或大骨缺损者普遍采用的治疗方法，但治疗周期长，并发症发生率高。DO过程中良好的骨形成对于减少治疗时间和减少相关并发症至关重要。我们认为DO是研究成骨生物学的良好模型。组织工程策略包括三个基本组成部分(应用成骨细胞、骨诱导因子和支架)，已成为促进牵张缝隙中骨再生的可能选择。如果在地塞米松的存在下培养，骨髓间充质干细胞(BMC)可以定向向成骨方向分化。即使在hrBMP-2存在的情况下，经过多次传代的BMSCs也可以保持成骨能力。富血小板血浆(Prp)含有多种骨诱导生长因子，包括pla…。更多的端粒源性生长因子、胰岛素样生长因子和转化生长因子。PRP能增加BMC的数量，但不能促进BMC的成骨能力。一些研究人员报道，将细胞注入牵张的骨痂可以促进骨形成，然而，注入的细胞可能会移动到周围组织，导致靶区浓度降低。因此，脚手架是重要的影响因素。根据我们的研究，我们得出结论，PRP是一种适合细胞移植的载体，因为它可以通过添加凝血酶和钙而立即凝固。负重和运动可能是DO成骨的另一个因素。我们发现，与非负重状态相比，牵张腿的负重状态可以刺激骨形成。对于基于BMC的治疗的临床应用，自体PRP被用来防止疾病传播和免疫反应。在DO期间移植BMC和PRP可能是一种很有前途的方法。较少

Distraction osteogenesis (DO) has become a widely used and accepted procedure in patients with limb length discrepancy or large bone defect, but the treatment period is long which results in higher rates of complications. Good bone formation during DO is essential to minimize the treatment time and reduce associated complications. We considered that DO is good model for investigating the biology of osteogensis. Tissue engineering strategy, which consists of three essential components (application of osteogenic cells, osteoinductive factors, and a scaffold), has emerged as a possible alternative to accelerate bone regeneration at the distracted gap.Bone marrow-derived mesenchymal stem cells(BMC)can be directed towards the osteogenic lineage if cultured in the presence of dexamethasone. Osteogenic potential can be maintained even in BMSCs that have been passaged several times in the presence of hrBMP-2. Platelet rich plasma(PRP)contains several osteoinductive growth factors including pla … More telet-derived growth factor, insulin-like growth factor, and transforming growth factors. PRP increased the number of BMC, but did not promote osteogenic potential in cultured BMC cells. Several investigators have reported that the injection of cells into the distracted callus enhanced bone formation However, the injected cells may move to the surrounding tissue, leading to a reduced concentration in the target area. Therefore, the scaffold is important factor. We concluded PRP can be a suitable carrier for cell transplantation because it coagulates immediately by an addition of thrombin and calcium from our studies. The weight bearing and movement may be another factor to develop osteogenesis in DO. We revealed that the weight bearing with distracted leg stimulated bone formation compared to non-weight bearing condition.For the clinical application of BMC based therapy, autologous PRP was used to prevent disease transmission and immunoreactions. The transplantation of BMC and PRP during DO may be regarded as promising procedure. Less