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The development of tissue engineering procedure for osteogensesis -investigation using plate rich plasma and mesenchymal stem cell-

成骨组织工程程序的开发 - 使用富含平板的血浆和间充质干细胞进行研究 -

基本信息

批准号：
17390416
负责人：
ISHIGURO Naoki
金额：
$ 9.63万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

项目摘要

Distraction osteogenesis (DO) has become a widely used and accepted procedure in patients with limb length discrepancy or large bone defect, but the treatment period is long which results in higher rates of complications. Good bone formation during DO is essential to minimize the treatment time and reduce associated complications. We considered that DO is good model for investigating the biology of osteogensis. Tissue engineering strategy, which consists of three essential components (application of osteogenic cells, osteoinductive factors, and a scaffold), has emerged as a possible alternative to accelerate bone regeneration at the distracted gap.Bone marrow-derived mesenchymal stem cells(BMC)can be directed towards the osteogenic lineage if cultured in the presence of dexamethasone. Osteogenic potential can be maintained even in BMSCs that have been passaged several times in the presence of hrBMP-2. Platelet rich plasma(PRP)contains several osteoinductive growth factors including pla … More telet-derived growth factor, insulin-like growth factor, and transforming growth factors. PRP increased the number of BMC, but did not promote osteogenic potential in cultured BMC cells. Several investigators have reported that the injection of cells into the distracted callus enhanced bone formation However, the injected cells may move to the surrounding tissue, leading to a reduced concentration in the target area. Therefore, the scaffold is important factor. We concluded PRP can be a suitable carrier for cell transplantation because it coagulates immediately by an addition of thrombin and calcium from our studies. The weight bearing and movement may be another factor to develop osteogenesis in DO. We revealed that the weight bearing with distracted leg stimulated bone formation compared to non-weight bearing condition.For the clinical application of BMC based therapy, autologous PRP was used to prevent disease transmission and immunoreactions. The transplantation of BMC and PRP during DO may be regarded as promising procedure. Less

牵张成骨术（Distraction osteogenesis，DO）已成为治疗肢体不等长或大面积骨缺损的广泛应用和接受的手术，但治疗周期长，并发症发生率高。DO期间良好的骨形成对于减少治疗时间和减少相关并发症至关重要。我们认为DO是研究成骨生物学的良好模型。组织工程技术是促进骨缺损再生的一种新方法，它包括成骨细胞、骨诱导因子和支架材料的应用，骨髓间充质干细胞（BMC）在地塞米松的作用下可以定向分化为成骨细胞。即使在hrBMP-2存在下传代数次的BMSC中，也可以保持成骨潜力。富血小板血浆（PRP）含有几种骨诱导生长因子，包括血小板 ...更多信息 telet衍生生长因子、胰岛素样生长因子和转化生长因子。PRP增加BMC的数量，但不促进培养的BMC细胞的成骨潜能。一些研究人员报告说，将细胞注射到分散的骨痂中可以增强骨形成，然而，注射的细胞可能会移动到周围组织，导致目标区域的浓度降低。因此，支架是重要因素。我们得出结论，PRP可以是一个合适的载体细胞移植，因为它立即凝固，从我们的研究中添加凝血酶和钙。负重和运动可能是DO发生的另一个因素。结果显示，与非负重状态相比，负重状态下骨形成明显增加，自体PRP可有效预防疾病传播和免疫反应，促进BMC治疗的临床应用。在DO期间移植BMC和PRP可能被认为是有希望的方法。少