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Establishment of canine iPS cells for the study of disease-model dogs

建立犬 iPS 细胞用于研究疾病模型犬

基本信息

批准号：
24659675
负责人：
TOGUCHIDA Junya
金额：
$ 2.33万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Challenging Exploratory Research
财政年份：
2012
资助国家：
日本
起止时间：
2012-04-01 至 2014-03-31
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659675/
关键词：
iPS細胞イヌ疾患モデル

项目摘要

To establish canine iPS cells, canine four reprogramming factor were introduced into canine embryonic fibroblasts with the EOS vector system, which allowed us to select clones with the expression of endogenous Oct3/4 gene by drug-resistance and fluorescence of GFP. Selected clones were positive for stem cell marker genes and able to make embryoid bodies, which showed the induction of marker genes specific for each of three germ layers. Teratoma formation was, however, not observed. Using chemically defined medium, these clones were maintained at undifferentiated state during several passages, but the long term culture forced the spontaneous differentiation of these clones, and therefore no clones with the feature compatible with iPS cells were established. These data indicated that more investigation will be required to find the suitable culture condition for canine iPS cells.

为了建立犬iPS细胞，利用EOS载体系统将犬4重编程因子导入犬胚胎成纤维细胞，通过耐药性和GFP荧光检测筛选出内源性Oct 3/4基因表达的克隆。选择的克隆是阳性的干细胞标记基因，并能够使胚状体，这表明诱导的标记基因特异性的三个胚层。然而，未观察到畸胎瘤形成。使用化学成分确定的培养基，这些克隆在几代中保持未分化状态，但长期培养迫使这些克隆自发分化，因此没有建立具有与iPS细胞相容的特征的克隆。这些数据表明，将需要更多的研究，以找到适合犬iPS细胞的培养条件。

项目成果

期刊论文数量（28）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Generation of canine iPS cells and their characteristics.

犬 iPS 细胞的产生及其特性。

DOI：
发表时间：
期刊：
影响因子：
0
作者：
早川和男;加藤友久;那須輝;池谷真;堀田秋津;大塚隆信;戸口田淳也．
通讯作者：
戸口田淳也．

Induced pluripotent stem cells from patients with human fibrodysplasia ossificans progressiva show increased mineralization and cartilage formation.

DOI：
10.1186/1750-1172-8-190
发表时间：
2013-12-09
期刊：
Orphanet journal of rare diseases
影响因子：
3.7
作者：
Matsumoto Y;Hayashi Y;Schlieve CR;Ikeya M;Kim H;Nguyen TD;Sami S;Baba S;Barruet E;Nasu A;Asaka I;Otsuka T;Yamanaka S;Conklin BR;Toguchida J;Hsiao EC
通讯作者：
Hsiao EC

Understanding the pathology of the arthropathy in chronic infantile neurological cutaneous and articular syndrome by using iPS cells technology

利用 iPS 细胞技术了解慢性婴儿神经皮肤和关节综合征关节病的病理学