Establishment of mouse model of synovial sarcomas using lineage-specific gene targeting system

利用谱系特异性基因打靶系统建立小鼠滑膜肉瘤模型

• 批准号: 21390420
• 负责人: TOGUCHIDA Junya
• 金额: $ 11.07万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390420/
• 关键词: 滑膜肉腫; 神経堤; 融合遺伝子; ノックインマウス

To prove out hypothesis that cell-of-origin of synovial sarcomas(SS) is neural crest derived cells, we established transgenic mice in which the expression of SS-specific fusion gene, SYT-SSX was induced in neural crest cells using the P0 gene as a driver. The expression of fusion gene in utero inhibited the development of craniofacial bone and cartilage tissues causing stillbirth. Then we established inducible transgenic mice using tamoxifen-ER system. Treatment with tamoxifen successfully induced the expression of SYT-SSX and also its downstream gene in P0 positive cells, indicating that this mouse model is a useful tool to understand the development of synovial sarcomas.

为了证明滑膜肉瘤（SS）的细胞起源是神经嵴衍生细胞的假设，我们建立了转基因小鼠，其中使用P0基因作为驱动因子，在神经嵴细胞中诱导SS特异性融合基因SYT-SSX的表达。融合基因在子宫内的表达抑制颅面骨和软骨组织的发育，导致死产。然后我们利用他莫昔芬-ER系统建立了诱导型转基因小鼠。他莫昔芬治疗成功诱导 P0 阳性细胞中 SYT-SSX 及其下游基因的表达，表明该小鼠模型是了解滑膜肉瘤发展的有用工具。

项目成果

Prostaglandin E2 receptor type 2-selective agonist prevents the degeneration of articular cartilage in rabbit knees with traumatic instability.

• DOI: 10.1186/ar3460
• 发表时间: 2011
• 期刊: Arthritis research & therapy
• 影响因子: 4.9
• 作者: Mitsui H;Aoyama T;Furu M;Ito K;Jin Y;Maruyama T;Kanaji T;Fujimura S;Sugihara H;Nishiura A;Otsuka T;Nakamura T;Toguchida J
• 通讯作者: Toguchida J

Gene Expression Profiling of Synovial Sarcoma: Distinct Signature of Poorly Differentiated Type

• DOI: 10.1097/pas.0b013e3181f7ce2c
• 发表时间: 2010-11-01
• 期刊: AMERICAN JOURNAL OF SURGICAL PATHOLOGY
• 影响因子: 5.6
• 作者: Nakayama, Robert;Mitani, Sachiyo;Ichikawa, Hitoshi
• 通讯作者: Ichikawa, Hitoshi

癌化と再生の接点としての組織幹細胞

组织干细胞作为癌化和再生之间的联系点

• 发表时间: 2010
• 作者: Tatsumi E;Katagiri N;Takewa Y;Mizuno T;Tsukiya T;Homma A;Taenaka Y;Hayashi T;Yagihara T;戸口田淳也
• 通讯作者: 戸口田淳也

網羅的発癌解析からの骨肉腫造腫瘍能の探索

通过综合致癌分析探索骨肉瘤的致瘤潜力

• 发表时间: 2010
• 作者: 笠原崇、戸口田淳也;他
• 通讯作者: 他

Involvement of cancer biomarker C7orf24 in the growth of human osteosarcoma.

• 发表时间: 2011-04
• 期刊: Anticancer research
• 影响因子: 2
• 作者: D. Uejima;K. Nishijo;Y. Kajita;T. Ishibe;T. Aoyama;S. Kageyama;H. Iwaki;Takashi Nakamura;H. Iida;T. Yoshiki;J. Toguchida