Establishment of mouse model of synovial sarcomas using lineage-specific gene targeting system

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To prove out hypothesis that cell-of-origin of synovial sarcomas(SS) is neural crest derived cells, we established transgenic mice in which the expression of SS-specific fusion gene, SYT-SSX was induced in neural crest cells using the P0 gene as a driver. The expression of fusion gene in utero inhibited the development of craniofacial bone and cartilage tissues causing stillbirth. Then we established inducible transgenic mice using tamoxifen-ER system. Treatment with tamoxifen successfully induced the expression of SYT-SSX and also its downstream gene in P0 positive cells, indicating that this mouse model is a useful tool to understand the development of synovial sarcomas.