Identification of endometrial cancer stem cell markers

子宫内膜癌干细胞标志物的鉴定

• 批准号: 24659736
• 负责人: KATO Kiyoko
• 金额: $ 2.16万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659736/
• 关键词: 子宮体癌; 癌幹細胞; バイオマーカー

We analyzed the association with dbpC/contrin(dbpC) and the character of endometrial cancer stem cells .We designed and constructed a cytomegarovirus vector containing dbpC cDNA, and transfected it into Ishikawa(IK) cells, an endometrial cancer cell line. The expression of ALDH1 was increased in IK-dbpC cells, compared to mock cells,and the ratio of SP cells were more than 10-fold in IK-dbpC cells compared to mock cells . Downreguration of dbpC expression was remarkably reduced the ALDH1 expression and the rate of SP cells, compared to negative control. Microarray analysis showed that one of the cancer/testis antigens was up-regulated in IK-dbpC cells compared with mock cells.

为了研究dbpC/contrin（dbpC）基因与子宫内膜癌干细胞特性的关系，设计并构建了含有dbpC cDNA的巨细胞病毒载体，转染子宫内膜癌细胞系石川（IK）。 IK-dbpC细胞中ALDH 1的表达较mock细胞增加，SP细胞的比例是mock细胞的10倍以上。与阴性对照组相比，dbpC表达下调可显著降低ALDH 1的表达和SP细胞的比例。 微阵列分析显示， 与模拟细胞相比，一种癌/睾丸抗原在IK-dbpC细胞中上调。

项目成果

産婦人科における幹細胞研究

干细胞在妇产科的研究

• 作者: Shintaku H;Inaoka T;Nakagawa T;Kawano S;Ito J.;加藤聖子
• 通讯作者: 加藤聖子

Effect of transient TCDD exposure on immortalized human trophoblast-derived cell lines

• DOI: 10.1177/0960327111424305
• 发表时间: 2012-06-01
• 期刊: HUMAN & EXPERIMENTAL TOXICOLOGY
• 影响因子: 2.8
• 作者: Fukushima, K.;Tsukimori, K.;Wake, N.
• 通讯作者: Wake, N.

子宮内膜、子宮体癌幹細胞の同定と生物学的特性の解析

子宫内膜及子宫内膜癌干细胞生物学特性的鉴定与分析

• 发表时间: 2013
• 作者: Yusuf N;Inagaki T;Kusunoki S;Okabe H;Yamada I;Matsumoto A;Terao T;Takeda S;Kato K;Kiyoko Kato;加藤聖子;加藤聖子
• 通讯作者: 加藤聖子

The inhibitory effect of salinomycin on the proliferation, migration and invasion of human endometrial cancer stem-like cells

• DOI: 10.1016/j.ygyno.2013.03.005
• 发表时间: 2013-06-01
• 期刊: GYNECOLOGIC ONCOLOGY
• 影响因子: 4.7
• 作者: Kusunoki, Soshi;Kato, Kiyoko;Takeda, Satoru
• 通讯作者: Takeda, Satoru

[子宮体癌の生物学的特性から診断、治療まで]癌遺伝子と癌幹細胞

【从生物学特性到子宫内膜癌的诊治】癌基因与癌干细胞

• 发表时间: 2012
• 期刊: 産科と婦人科
• 作者: Fukushima K;Tsukimori K;Li D;Takao T;Morokuma S;Kato K;Seki H;Takeda S;Matsumura S;Wake N;Kato K;Kato K;加藤聖子
• 通讯作者: 加藤聖子