Elucidation of the in vivo migration of mesenchymal stem cells.

阐明间充质干细胞的体内迁移。

• 批准号: 24659789
• 负责人: MATSUZAKI Yumi
• 金额: $ 2.33万
• 依托单位: Tokyo Medical University (2013)Keio University (2012)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659789/
• 关键词: 間葉系幹細胞; 創傷治癒学

We report an improved prospective clonal isolation technique and reveal that the combination of three cell-surface markers (LNGFR, THY-1, and VCAM-1) allows for the selection of highly enriched clonogenic cells (one out of three isolated cells). Clonal characterization of LNGFR+THY-1+ cells demonstrated cellular heterogeneity among the clones. Rapidly expanding clones (RECs) exhibited robust multilineage differentiation and self-renewal potency, whereas the other clones tended to acquire cellular senescence via P16INK4a and exhibited frequent genomic errors. Furthermore, RECs exhibited unique expression of VCAM-1 and higher cellular motility compared with the other clones. The combination marker LNGFR+THY-1+VCAM-1hi+ (LTV) can be used selectively to isolate the most potent and genetically stable MSCs.

我们报告了一种改进的前瞻性克隆分离技术，并揭示了三种细胞表面标志物（LNGFR，THY-1和VCAM-1）的组合允许选择高度富集的克隆细胞（三个分离的细胞中的一个）。LNGFR+THY-1+细胞的克隆表征证实了克隆之间的细胞异质性。快速扩增克隆（RECs）表现出强大的多系分化和自我更新能力，而其他克隆往往通过P16 INK 4a获得细胞衰老，并表现出频繁的基因组错误。此外，与其他克隆相比，RECs表现出独特的VCAM-1表达和更高的细胞运动性。组合标记LNGFR+THY-1+VCAM-1hi+（LTV）可选择性地用于分离最有效和遗传稳定的MSC。

项目成果

Kidney specific protein-positive cells derived from embryonic stem cells reproduce tubular structures in vitro and differentiate into renal tubular cells.

• DOI: 10.1371/journal.pone.0064843
• 发表时间: 2014
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Morizane R;Monkawa T;Fujii S;Yamaguchi S;Homma K;Matsuzaki Y;Okano H;Itoh H
• 通讯作者: Itoh H

超高純度ヒト間葉系幹細胞の解析『遊走能と未分化性維持』

超高纯度人间充质干细胞“迁移能力及未分化状态维持”分析

• 发表时间: 2013
• 作者: Mabuchi Y;Houlihan DD;Okano H and Matsuzaki Y;松崎有未;松崎有未;松崎有未
• 通讯作者: 松崎有未

Adipose stromal cells contain phenotypically distinct adipogenic progenitors derived from neural crest.

• DOI: 10.1371/journal.pone.0084206
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Sowa Y;Imura T;Numajiri T;Takeda K;Mabuchi Y;Matsuzaki Y;Nishino K
• 通讯作者: Nishino K

超高純度ヒトMSCの分離とその機能解析

超纯人间充质干细胞的分离及其功能分析

• 发表时间: 2014
• 作者: Mabuchi Y;Houlihan DD;Okano H and Matsuzaki Y;松崎有未
• 通讯作者: 松崎有未

間葉系幹細胞の体内動態と慢性GVHD誘導

间充质干细胞药代动力学和慢性 GVHD 诱导

• 发表时间: 2014
• 作者: Mabuchi Y;Houlihan DD;Okano H and Matsuzaki Y;松崎有未;松崎有未
• 通讯作者: 松崎有未