Visulization of Wnt/Notch signaling for the maintenance of mesenchymal stem cells

用于维持间充质干细胞的 Wnt/Notch 信号传导的可视化

• 批准号: 21390062
• 负责人: MATSUZAKI Yumi
• 金额: $ 11.98万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390062/
• 关键词: 間葉系幹細胞; 再生医学; 細胞・組織; 移植・再生医療; 間薬系幹細胞

It has been advocated that Wnt, Notch signals involve molecular mechanism of self-renewal or differentiation in various somatic stem cells. In mammalian development, stem cell controls complicated organizational structure as high-order function, while controlling self-renew and differentiation. In this project, we attempted to clarify self-renewal and cell lineage decision mechanism of MSC. Our method was to visualize Wnt, Notch signals, which has a potential for temporospatial controls of stem cells. By visualizing Wnt, Notch signals, we could analyze how Wnt/Notch separates their roles, or interacts with other molecules in order for self-replication.

Wnt、Notch信号参与了多种体细胞干细胞自我更新或分化的分子机制。在哺乳动物发育过程中，干细胞作为高阶功能控制着复杂的组织结构，同时控制着自我更新和分化。在本项目中，我们试图阐明MSC的自我更新和细胞谱系决定机制。我们的方法是可视化Wnt， Notch信号，它具有干细胞时空控制的潜力。通过可视化Wnt， Notch信号，我们可以分析Wnt/Notch如何分离它们的角色，或与其他分子相互作用以进行自我复制。

项目成果

Stem cell-like properties of the endometrial side population: implication in endometrial regeneration.

• DOI: 10.1371/journal.pone.0010387
• 发表时间: 2010-04-28
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Masuda H;Matsuzaki Y;Hiratsu E;Ono M;Nagashima T;Kajitani T;Arase T;Oda H;Uchida H;Asada H;Ito M;Yoshimura Y;Maruyama T;Okano H
• 通讯作者: Okano H

Bidirectional Signaling through EphrinA2-EphA2 Enhances Osteoclastogenesis and Suppresses Osteoblastogenesis

• DOI: 10.1074/jbc.m807598200
• 发表时间: 2009-05-22
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Irie, Naoko;Takada, Yasunari;Matsuo, Koichi
• 通讯作者: Matsuo, Koichi

Cell surface N-glycans mediated isolation of mouse neural stem cells

• DOI: 10.1111/j.1471-4159.2009.06256.x
• 发表时间: 2009-09-01
• 期刊: JOURNAL OF NEUROCHEMISTRY
• 影响因子: 4.7
• 作者: Hamanoue, Makoto;Matsuzaki, Yumi;Okano, Hideyuki
• 通讯作者: Okano, Hideyuki

ヒト子宮内膜幹様細胞の同定. 分離とその幹細胞特性の機能解析.

人类子宫内膜干细胞的鉴定及其干细胞特性的功能分析。

• 发表时间: 2008
• 作者: 升田博隆;丸山哲夫;小野政徳;長島 隆;梶谷 宇;内田 浩;浅田弘法;岡野栄之;松崎有未;吉村泰典
• 通讯作者: 吉村泰典

Direct isolation system of human mesenchymal stem cells by FCM

FCM直接分离人间充质干细胞系统

• 发表时间: 2009
• 作者: 安西尚彦;内田幸介;林啓太朗;松崎有未
• 通讯作者: 松崎有未