Is epithelial-mesenchymal transition of stellate reticulum cells in enamel organ associated with induction of vasculogenesis?

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In the late stage of tooth germ development, vasculogenesis is observed in the enamel organ. To discover the mechanisms of the vasculogenesis, we studied on the relationship between the vasculogenesis and epithelial-mesenchymal transition using Keratin14 cre-b-gal transgenic mouse, Mesp1 cre-b-gal transgenic mouse and Flk1-GFP transgenic mouse. Also, the cell dynamics of enamel organ was observed by real-time imaging of tooth germ organ culture. The study showed that the development of stellate reticulum cells is derived from inner enamel epithelial cells, and that the vasculogenesis and epithelial-mesenchymal transition is closely associated with hypoxia in the enamel organ from expression of Hif1. Furthermore, it is considered that the vasculogenesis is related with ameloblast differentiation to calcify the enamel matrix.