Is epithelial-mesenchymal transition of stellate reticulum cells in enamel organ associated with induction of vasculogenesis?

釉质器官中星状网状细胞的上皮-间质转化与血管生成的诱导有关吗？

• 批准号: 24659818
• 负责人: HARADA Hidemitsu
• 金额: $ 2.33万
• 依托单位: Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659818/
• 关键词: 歯の発生; 血管新生; 上皮間葉転換; エナメル器; 星状網; 血管構築

In the late stage of tooth germ development, vasculogenesis is observed in the enamel organ. To discover the mechanisms of the vasculogenesis, we studied on the relationship between the vasculogenesis and epithelial-mesenchymal transition using Keratin14 cre-b-gal transgenic mouse, Mesp1 cre-b-gal transgenic mouse and Flk1-GFP transgenic mouse. Also, the cell dynamics of enamel organ was observed by real-time imaging of tooth germ organ culture. The study showed that the development of stellate reticulum cells is derived from inner enamel epithelial cells, and that the vasculogenesis and epithelial-mesenchymal transition is closely associated with hypoxia in the enamel organ from expression of Hif1. Furthermore, it is considered that the vasculogenesis is related with ameloblast differentiation to calcify the enamel matrix.

在牙胚发育的后期，在釉器官中观察到血管发生。为了揭示血管发生的机制，我们采用Keratin 14 cre-b-gal转基因小鼠、Mesp 1 cre-b-gal转基因小鼠和Flk 1-GFP转基因小鼠，研究了血管发生与上皮-间质转化的关系。同时，通过牙胚器官培养的实时成像技术，观察成釉器细胞的动态变化。研究表明，星状网状细胞的发育起源于内釉上皮细胞，而成釉器中HIF 1的表达与血管发生和上皮-间充质转化密切相关。此外，认为成釉细胞的分化与釉基质钙化有关。

项目成果

Promotional effects of vasoactive intestinal peptide on the development of rodent Hertwig’s epithelial root sheath

血管活性肠肽对啮齿动物Hertwig上皮根鞘发育的促进作用

• DOI: 10.1111/j.1741-4520.2012.00371.x
• 发表时间: 2012
• 期刊: Congenital Anomalies
• 影响因子: 1.3
• 作者: J Xu;N Kawashima;N Fujiwara;H Harada;MS. Ota;H Suda
• 通讯作者: H Suda

Role of Rho signaling during amelogenesis. Tripartite Conference (Korea- Chaina- Japan) on Tooth and Bone; Development & Regeneration" in Seoul

Rho 信号在釉质形成过程中的作用。

• 发表时间: 2013
• 作者: Harada;H.;Otsu;K.;Sakano;M.;Fujiwara;N
• 通讯作者: N

口腔領域におけるiPS細胞研究の現状と展望

口腔领域iPS细胞研究现状及展望

• 发表时间: 2012
• 作者: 原田英光;江草 宏
• 通讯作者: 江草 宏

ライブイメージングでとらえる歯の発生メカニズム

通过实时成像捕捉牙齿发育机制

• 发表时间: 2012
• 作者: 大津圭史;原田英光
• 通讯作者: 原田英光

Circadian rhythms regulate amelogenesis.

• DOI: 10.1016/j.bone.2013.02.011
• 发表时间: 2013-07
• 期刊: BONE
• 影响因子: 4.1
• 作者: Zheng, Li;Seon, Yoon Ji;Mourao, Marcio A.;Schnell, Santiago;Kim, Doohak;Harada, Hidemitsu;Papagerakis, Silvana;Papagerakis, Petros
• 通讯作者: Papagerakis, Petros