Study on mechanisms of odontoblasts processes formation and dentin development

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We designed the real time imaging system in a slice tooth germ culture of actin promoter GFP transgenic mouse. Using the technique, we could capture the cell division and movement of mesenchymal cells in the dental papilla and demonstrate the formation of odontoblasts processes. We examined the role of Rho-associated kinase (ROCK) in odontoblasts differentiation using an organ culture of mouse incisor and mouse dental mesenchaml cell line. ROCK inhibitors and ROCK siRNA inhibited actin polymerization and E-cadherin expression on the cytoplasmic membrane. ROCK plays an important role in maintenance of cellular polarization through regulations of cytoskeleton and cell adhesion in the odontoblasts differentiation. Odotoblasts expressed the amelogenin, MMP inhibitors and MMP. Our data suggested that these factors play an important role in the maintenance of dentin canal.