Cellular mechanism of cardio-adrenal interaction

心-肾上腺相互作用的细胞机制

• 批准号: 25670396
• 负责人: SANO Motoaki
• 金额: $ 2.41万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2013
• 资助国家: 日本
• 起止时间: 2013-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-25670396/
• 关键词: 循環器・高血圧; 細胞・組織; 分析科学; 心不全; 副腎; レニン・アンジオテンシン系; 虚血; アルドステロン

Chronic salt overload in Dahl-salt sensitive rats stimulates aldosterone production via local activation of renin-angiotensin system (RAS). Beta3-adrenergic receptor was highly upregulated concomitant with the progression of cardio-renal injury. Adrenal glands became hypoxic as cardio-renal injury progress and both hypoxia induced the expression of beta3-adrenergic receptor via HIF activation in adrenocortical cells. Macrophages were infiltrated in the adrenal cortex in rats with cardio-renal injury and macrophage ablation reduced aldosterone production. Co-culture with macrophages or a supernatant of macrophages stimulated CYP11B2 expression in adrenocortical cells. Acquisition of catecholamine-responsiveness via the induction of beta3-adrenergic receptor under tissue ischemic and crosstalk between macrophages and adrenocortical cells accounts for the RAS-independent aldosterone production associated with cardio-renal injury.

Dahl盐敏感大鼠的慢性盐超负荷通过局部激活肾素-血管紧张素系统（RAS）刺激醛固酮产生。β 3-肾上腺素能受体随着心肾损伤的进展而高度上调。随着心肾损伤的进展，肾上腺变得缺氧，并且这两种缺氧都通过HIF激活肾上腺皮质细胞中的β 3肾上腺素能受体来诱导β 3肾上腺素能受体的表达。心肾损伤大鼠肾上腺皮质有巨噬细胞浸润，巨噬细胞消融减少了醛固酮的产生。与巨噬细胞或巨噬细胞上清液共培养刺激肾上腺皮质细胞中CYP 11 B2的表达。在组织缺血和巨噬细胞和肾上腺皮质细胞之间的串扰下，通过诱导β 3-肾上腺素能受体获得儿茶酚胺反应性，解释了与心肾损伤相关的RAS非依赖性醛固酮产生。

项目成果

The Unexpected Mechanism of Aldosterone Production in Salt-sensitive Dahl rats with Heart Failure

盐敏感 Dahl 心力衰竭大鼠产生醛固酮的意外机制

• 发表时间: 2013
• 作者: Kentaro Ito;Fumiko Mitani;Shintaro Morizane;Tomohiro Matsuhashi;Yoshinori Katsumata;Xiaoxiang Yan;Tsunehisa Yamamoto;Nobuhito Goda;Keiichi Fukuda;Motoaki Sano
• 通讯作者: Motoaki Sano

CIRCULATION Up-to-Date 誌

流通最新杂志

• 发表时间: 2013
• 作者: 佐野元昭;松橋智弘;勝俣良紀;伊藤健太郎
• 通讯作者: 伊藤健太郎

Emergence of β3-adrenergic Receptor and Macrophage Infiltration in the Adrenal Glands Contributed to the Unfavorable Aldosterone Production in Heart Failure

肾上腺β3-肾上腺素能受体的出现和巨噬细胞浸润导致心力衰竭时不利的醛固酮产生

• 发表时间: 2013
• 作者: Kentaro Ito;Fumiko Mitani;Shintaro Morizane ;Tomohiro Matsuhashi;Yoshinori Katsumata;Xiaoxiang Yan;Tsunehisa Yamamoto;Nobuhito Goda;Keiichi Fukuda;Motoaki Sano
• 通讯作者: Motoaki Sano

The Unexpected Mechanism of Aldosterone Production in Salt-sensitive Dahl rats with Heart Failure.

患有心力衰竭的盐敏感 Dahl 大鼠中醛固酮产生的意外机制。

• 发表时间: 2013
• 作者: Kentaro Ito;Fumiko Mitani;Shintaro Morizane ;Tomohiro Matsuhashi;Yoshinori Katsumata;Xiaoxiang Yan;Tsunehisa Yamamoto;Nobuhito Goda;Keiichi Fukuda;Motoaki Sano
• 通讯作者: Motoaki Sano

心不全期副腎におけるマクロファージの浸潤とアルドステロン産生異常

心力衰竭期间肾上腺巨噬细胞浸润和醛固酮异常产生

• 发表时间: 2013
• 作者: Kentaro Ito;Fumiko Mitani;Shintaro Morizane ;Tomohiro Matsuhashi;Yoshinori Katsumata;Xiaoxiang Yan;Tsunehisa Yamamoto;Nobuhito Goda;Keiichi Fukuda;Motoaki Sano;伊藤健太郎
• 通讯作者: 伊藤健太郎