The regulatory role of five small noncoding RNAs in Streptococcus pneumoniae

5种小非编码RNA在肺炎链球菌中的调控作用

• 批准号: 39768282
• 负责人: Privatdozent Dr. Reinhold Brückner
• 金额: --
• 依托单位: Fachgebiet Mikrobiologie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/39768282?language=en
• 关键词: regulatory; role; five; small; noncoding

The two component regulatory system CiaRH of Streptococcus pneumoniae is implicated in ß-lactam resistance, lytic processes, genetic competence, and virulence. The CiaR regulon consists of 24 genes including five small non-coding RNAs (csRNAs). A first search for csRNA targets revealed three genes. Two of them, comC and spr0081, are downregulated by the csRNAs in the wild type strain, while the third, comA, is regulated only in a strain overexpressing all csRNAs. In this strain, genetic competence is blocked as a consequence of com gene repression by csRNA overproduction. In addition, csRNAs are implicated in ß-lactam resistance, but their molecular targets in this process are not yet known. In the project applied here, phenotypic consequences of the established csRNA regulations will be explored in further detail. The role of individual csRNAs in the regulatory process and the contribution of single residues in csRNAs to target binding will be analyzed. To find new targets and interaction partners of the csRNAs, bioinformatics, proteome analysis, and a cDNA deep sequencing approach will be pursued. New regulatory interactions will be evaluated by in vivo regulation studies and in vitro csRNA binding assays. Defining new targets and regulatory processes should provide a comprehensive view on csRNA activities and their impact on the physiology of S. pneumoniae.

肺炎链球菌的双组分调节系统CiaRH涉及β-内酰胺抗性、裂解过程、遗传能力和毒力。CiaR调节子由24个基因组成，包括5个小的非编码RNA（csRNA）。对csRNA靶点的首次搜索发现了三个基因。其中两个，comC和spr 0081，在野生型菌株中被csRNA下调，而第三个，comA，仅在过表达所有csRNA的菌株中被调节。在该菌株中，由于csRNA过量产生的玉米基因阻遏，遗传能力被阻断。此外，csRNA与β-内酰胺抗性有关，但其在该过程中的分子靶点尚不清楚。在这里应用的项目中，将进一步详细探索已建立的csRNA调控的表型后果。将分析单个csRNA在调控过程中的作用以及csRNA中单个残基对靶结合的贡献。为了找到新的目标和相互作用的合作伙伴的csRNA，生物信息学，蛋白质组分析，和cDNA深度测序的方法将被追求。将通过体内调控研究和体外csRNA结合试验评价新的调控相互作用。定义新的靶标和调控过程应该提供对csRNA活性及其对S.肺炎。