Highly dynamic multianalyte assays - Simultaneous detection of nucleic acids and proteins in one go

今天，各种生物分析方法被用于检测不同类别的生物分子，如蛋白质或核酸。针对每类生物分子设计和优化特定的测定，并且是体外诊断、药物开发或食品安全等应用领域的重要工具。然而，在这些领域，同时分析几种不同类型的生物分子越来越重要。因此，将分析不同种类的生物分子如蛋白质和核酸的能力结合到一个单一的测定中将导致在劳动力和时间消耗方面的大规模简化的工作流程，并且可以省略一些专用设备的使用。如果蛋白质和核酸检测可以在一个单一的平台上进行，这将意味着巨大的经济进步。在“DFG Sachbeihilfe”的帮助下，将探索这种多分析物测定的创新概念。通过核酸信息到蛋白质信息的转换，仅用一种测量原理就可实现对不同类别生物分子的同时检测。基于用途：在医院伤口细菌感染的情况下，应在一个单一平台上探索使用核酸检测的病原体鉴定与使用蛋白质检测的宿主免疫应答（炎症）之间的相互作用。基于这种分析的改进的伤口管理可以促进对每个特定病例的快速和特异性干预以及更有效的抗生素治疗。这是非常有价值的，特别是对于抗生素耐药性的威胁。

Today, various bioanalytical methods are used to detect different classes of biomolecules like proteins or nucleic acids. Particular assays are designed and optimized for each class of biomolecules and are important tool in areas of application like in-vitro diagnostics, drug development or food safety. However, in these areas it is more and more important to analyze several different classes of biomolecules at once. Thus combining the capability of analyzing different classes of biomolecules like proteins and nucleic acids into one single assay would lead to a massively simplified workflow in terms of labor and time consumption as well as the use of some specialized equipment can be omitted. It would mean a substantial economic advance if protein and nucleic acid detection can be performed on one single platform. With the help of the "DFG Sachbeihilfe" an innovative concept for such a multianalyte assay shall be explored. Through the conversion of nucleic acid information into protein information, the simultaneous detection of different classes of biomolecules shall be realized using only one measurement principle. The characterization of this novel multianalyte assay approach and integration on a point-of-care platform is pursued on the basis of an example of use: In the case of bacterial infections of wounds in hospitals, the interplay between pathogen identification using nucleic acid detection and host immune response (inflammation) using protein detection shall be explored on one single platform. An improved wound management based on this analysis could facilitate fast and specific intervening in each particular case as well as a more efficient antibiotic therapy. This is of great value especially regarding the threat of antibiotic resistances.