权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Highly dynamic multianalyte assays - Simultaneous detection of nucleic acids and proteins in one go

高动态多分析物检测 - 一次性同时检测核酸和蛋白质

基本信息

批准号：
397660978
负责人：
Dr. Susanna Früh
金额：
--
依托单位：
Professur für Anwendungsentwicklung
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2018
资助国家：
德国
起止时间：
2017-12-31 至 2021-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/397660978?language=en
关键词：
Highly dynamic multianalyte assays Simultaneous

项目摘要

Today, various bioanalytical methods are used to detect different classes of biomolecules like proteins or nucleic acids. Particular assays are designed and optimized for each class of biomolecules and are important tool in areas of application like in-vitro diagnostics, drug development or food safety. However, in these areas it is more and more important to analyze several different classes of biomolecules at once. Thus combining the capability of analyzing different classes of biomolecules like proteins and nucleic acids into one single assay would lead to a massively simplified workflow in terms of labor and time consumption as well as the use of some specialized equipment can be omitted. It would mean a substantial economic advance if protein and nucleic acid detection can be performed on one single platform. With the help of the "DFG Sachbeihilfe" an innovative concept for such a multianalyte assay shall be explored. Through the conversion of nucleic acid information into protein information, the simultaneous detection of different classes of biomolecules shall be realized using only one measurement principle. The characterization of this novel multianalyte assay approach and integration on a point-of-care platform is pursued on the basis of an example of use: In the case of bacterial infections of wounds in hospitals, the interplay between pathogen identification using nucleic acid detection and host immune response (inflammation) using protein detection shall be explored on one single platform. An improved wound management based on this analysis could facilitate fast and specific intervening in each particular case as well as a more efficient antibiotic therapy. This is of great value especially regarding the threat of antibiotic resistances.

今天，各种生物分析方法被用来检测不同类别的生物分子，如蛋白质或核酸。特定的分析是针对每一类生物分子而设计和优化的，是体外诊断、药物开发或食品安全等应用领域的重要工具。然而，在这些领域中，同时分析几种不同类别的生物分子变得越来越重要。因此，将分析不同类别的生物分子(如蛋白质和核酸)的能力合并到一种分析方法中，将大大简化工作流程，节省人力和时间消耗，并且可以省去一些专门设备的使用。如果蛋白质和核酸检测可以在一个平台上进行，这将意味着实质性的经济进步。在“DFG Sachbeihelfe”的帮助下，将探索这样一种多分析分析的创新概念。通过将核酸信息转换为蛋白质信息，仅使用一种测量原理就可以实现对不同类别生物分子的同时检测。这种新的多分析分析方法的特征和在一个护理点平台上的集成是基于一个使用的例子：在医院伤口的细菌感染的情况下，使用核酸检测的病原体识别和使用蛋白质检测的宿主免疫反应(炎症)之间的相互作用应该在一个单一平台上探索。基于这一分析的改进的伤口处理可以促进对每个特定病例的快速和特定的干预，以及更有效的抗生素治疗。这是非常有价值的，特别是关于抗生素耐药性的威胁。