Development of new in vivo systems for the evaluation of growth factors function.

由于许多生长因子的最新发现，预期这些物质在动物工业中的实际利用用于动物功能的生理调节或预防由于疾病引起的浪费。为此，除了已被广泛用于研究这些物质的作用模式的常用细胞培养系统外，还应开发许多其他体内评价系统。该研究项目旨在开发新的体内系统，用于评价生长因子的整体功能，并进行试验，以发现生长因子功能的新方面。我们的研究结果包括：1）抗神经生长因子抗体可延缓杏仁核的形成; 2）肿瘤坏死因子-α可抑制下丘脑促性腺激素释放激素脉冲发生器的活性; 3）激活素可促进哺乳动物早期胚胎的发育; 4）激活素可促进垂体促卵泡激素产生细胞的分化; 5）转化生长因子是黄体分泌孕酮所必需的; 6）胸腺肽相关的生物肽，可有效地抑制巨噬细胞内钩端螺旋体的增殖，也可作为一种广泛的免疫调节剂。

As a consequence of recent findings of many growth factors, practical utilization of these substances is expected in animal industry either for physiological regulation of animal function or prevention of wastage due to diseases. For this purpose in addition to the usual cell culture system which has been intensively used for the investigation of the action mode of these substances, many other in vivo evaluation systems should be developed. This research project was designed to develop new in vivo systems for the evaluation of overall function of growth factors together with the trials for finding new aspects of the growth factor function. The followings are the exampels of our findings :1) Anti-nerve growth factor antibody delay the development of amygdaloid kindeking.2) Tumor necrosis factor-a suppresses the activity of gonadotropin-releasing hormone pulse genarator in hypothalamus.3) Activing promote the development of mammaian early embryo.4) Activin enhance the differentiation of follicle stimulating hormone producing cells in the pituitary.5) Transforming growth factor is required for progesterone secretion from the corpus luteum.6) a thymosin-related peptide, obiopeptide is effective either for preventing proliferation of leptospira in macrophages or as a wide range immuno-modulator.