Development of new in vivo systems for the evaluation of growth factors function.

开发用于评估生长因子功能的新体内系统。

• 批准号: 01860040
• 负责人: TAKAHASHI Michio
• 金额: $ 10.37万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01860040/
• 关键词: Growth Factors; Cytokine; Activin; Transforming Growth Factor-beta; Nerve growth factor; Tumor Necrosis Factor-alpha; basic Fibroblast Growth Factor; Thymosin-related peptide; TGF-β; 細胞成長因子; 初期胚発生停止; Erythroid differentiation factor; Nerve gro

As a consequence of recent findings of many growth factors, practical utilization of these substances is expected in animal industry either for physiological regulation of animal function or prevention of wastage due to diseases. For this purpose in addition to the usual cell culture system which has been intensively used for the investigation of the action mode of these substances, many other in vivo evaluation systems should be developed. This research project was designed to develop new in vivo systems for the evaluation of overall function of growth factors together with the trials for finding new aspects of the growth factor function. The followings are the exampels of our findings :1) Anti-nerve growth factor antibody delay the development of amygdaloid kindeking.2) Tumor necrosis factor-a suppresses the activity of gonadotropin-releasing hormone pulse genarator in hypothalamus.3) Activing promote the development of mammaian early embryo.4) Activin enhance the differentiation of follicle stimulating hormone producing cells in the pituitary.5) Transforming growth factor is required for progesterone secretion from the corpus luteum.6) a thymosin-related peptide, obiopeptide is effective either for preventing proliferation of leptospira in macrophages or as a wide range immuno-modulator.

由于最近许多生长因子的发现，这些物质有望在动物工业中得到实际利用，用于动物功能的生理调节或预防疾病造成的浪费。为此目的，除了已被广泛用于研究这些物质的作用方式的通常的细胞培养系统外，还应开发许多其他的体内评价系统。本研究项目旨在开发新的体内系统，以评估生长因子的整体功能，并进行试验，以发现生长因子功能的新方面。以下是我们发现的例子：1)抗神经生长因子抗体延缓杏仁核缔合的发展。2)肿瘤坏死因子-a抑制下丘脑促性腺激素释放激素脉冲发生器的活性。3)积极促进哺乳动物早期胚胎的发育。4)激活素促进垂体促卵泡激素产生细胞的分化。5)黄体分泌黄体酮需要转化生长因子。6)一种胸腺素相关肽，生物肽可以有效地阻止巨噬细胞中钩端螺旋体的增殖或作为一种广泛的免疫调节剂。

项目成果

E. Tabuchi, T. Ono, H. Nishijo and K. Torii: "Amino acid and NaCl appetite and LHA neuron responses of lysine-deficient rat." Physiology and Behavior. 49. 951-964 (1991)

E. Tabuchi、T. Ono、H. Nishijo 和 K. Torii：“赖氨酸缺乏大鼠的氨基酸和氯化钠食欲以及 LHA 神经元反应。”

H. Sakurai, Y. Fujii, Y. Maki, I. Igarashi, Y. Omata, A. Saito, Y. Ono, N. Suzuki: "In vitro and in vivo studies of the growth inhibitory effect of a newly synthesized peptide, Obiopeptide-1, on micebearing methylcholanthrene-induced murine tumours" Journ

H. Sakurai、Y. Fujii、Y. Maki、I. Igarashi、Y. Omata、A. Saito、Y. Ono、N. Suzuki：“新合成肽的生长抑制作用的体外和体内研究，

H.Sakurai: "In vitro and in vivo studies of the growth inhibitory effect of a newly synthesized peptide,Obiopeptide-1,on mice-bearing methylcholanthrene-induced murine tumours" Journal of Veterinary Medical Science. 53. 823-831 (1991)

H.Sakurai：“新合成的肽 Obiopeptide-1 对携带甲基胆蒽诱导的小鼠肿瘤的生长抑制作用的体外和体内研究”《兽医医学科学》杂志。

Y.Kawaguchi,T.Miyazawa,T.Tohya,E.Takahashi,T.Mikami: "Quantitication of teline immunodeticiency virus in a newly established teline Tーlymphoblastoid cell line(MYAー1 cells)" Archives of Virology. 111. 269-273 (1990)

Y.Kawaguchi、T.Miyazawa、T.Tohya、E.Takahashi、T.Mikami：“新建立的兔 T 淋巴母细胞系（MYA-1 细胞）中兔免疫缺陷病毒的定量”病毒学档案 111。 269 -273 (1990)

S.Matsuyama: "Splenic Macrophage enhancce Iuteotropic action of prolactin and luteinizing hormone in rat luteal cell cultures." Endocrinologia Japonica. 39. (1992)

S.Matsuyama：“脾巨噬细胞增强大鼠黄体细胞培养物中催乳素和黄体生成素的顺向作用。”