Analysis of reproductive processes by means of transgenic animals

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This Study was performed to elucidate new factors regulating reproductive processes by amalyzing reproductive function in transgenic animals, and following results were obtained.1) Precosious puberty occurred in transgenic rats expressing human growth hormone, suggesting that body weight is not a prerequisite factor for the induction of puberty. 2) By means of administration of macrophages in the spleen of pregnant mice, implantation window could be opened earlier. 3) The sperm obtained from mice carring a targeted mutation of the acrosin gene could penetrate the oocyte zona pellucida. 4) Proliferation and growth arrest in mouse primordal germ cell were shown to be autonomously regulated by means of clonal cultures. 5) High incidence of ultraviolet-B- or chemical-carcinogen-induced skin tumors was demonstrated in mice lacking the xeroderma pigmentosum group A gene. 6) Abnormality in differentiation of chorionic cells was found in mice carring a targeted mutation of the interferon gene. 7) It was revealed that one of two Ets-binding sites in the cytokerarin EndoA enhancer is essential for enhancer activity and bind to Ets-2 related proteins. 8) A periodic network of G protein subunits were shown to coexist with cytokeratin filament starfish oocytes.