Analysis of reproductive processes by means of transgenic animals

通过转基因动物分析生殖过程

• 批准号: 05304023
• 负责人: TAKAHASHI Michio
• 金额: $ 18.56万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05304023/
• 关键词: Transgenic animal; Gene targetting; Reproduction; Embryo manipulation; Embryo differentiation; Fertilization; Implantation; Gonad; トランスジェニック動物

This Study was performed to elucidate new factors regulating reproductive processes by amalyzing reproductive function in transgenic animals, and following results were obtained.1) Precosious puberty occurred in transgenic rats expressing human growth hormone, suggesting that body weight is not a prerequisite factor for the induction of puberty. 2) By means of administration of macrophages in the spleen of pregnant mice, implantation window could be opened earlier. 3) The sperm obtained from mice carring a targeted mutation of the acrosin gene could penetrate the oocyte zona pellucida. 4) Proliferation and growth arrest in mouse primordal germ cell were shown to be autonomously regulated by means of clonal cultures. 5) High incidence of ultraviolet-B- or chemical-carcinogen-induced skin tumors was demonstrated in mice lacking the xeroderma pigmentosum group A gene. 6) Abnormality in differentiation of chorionic cells was found in mice carring a targeted mutation of the interferon gene. 7) It was revealed that one of two Ets-binding sites in the cytokerarin EndoA enhancer is essential for enhancer activity and bind to Ets-2 related proteins. 8) A periodic network of G protein subunits were shown to coexist with cytokeratin filament starfish oocytes.

本研究通过分析转基因动物的生殖功能来阐明调控生殖过程的新因素，得到了以下结果：1)表达人生长激素的转基因大鼠发生了性前期，表明体重不是诱导青春期的先决条件。2)妊娠小鼠脾内注射巨噬细胞可提前打开着床窗。3)携带顶体酶基因定点突变的小鼠精子可穿透卵母细胞透明带。4)克隆培养对小鼠原始生殖细胞的增殖和生长停滞具有自主调节作用。5)在缺乏A组着色性干皮病基因的小鼠中，紫外线-B或化学致癌物诱发的皮肤肿瘤发生率较高。6)携带干扰素基因靶向突变的小鼠绒毛细胞分化异常。7)研究发现，细胞角蛋白EndoA增强子中的两个ETS结合位点中的一个对增强子活性和与ETS-2相关蛋白的结合是必不可少的。8)细胞角蛋白细丝与海星卵母细胞共存的G蛋白亚基周期性网络。

项目成果

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Koshimizu 等人：“视黄酸是体外小鼠原始生殖细胞的有效生长激活剂”发育生物学 168. 683-685 (1995)。

Kato Y,Tsunoda Y: "Effect the reduction of cytoplasm of mouse 2-cell embryos on blastomere formation timing and developmental ability in vitro and in vivo." Theriogenology. 41. 1483-1488 (1994)

Kato Y，Tsunoda Y：“小鼠 2 细胞胚胎细胞质的减少对卵裂球形成时间和体外和体内发育能力的影响。”

Suzuki H 他: "Developmental ability of zona-free mouse embryos is influenced by cell association at the 4-cell stage" Biolology of Reproduction. 53. 78-83 (1995)

Suzuki H 等人：“无透明带小鼠胚胎的发育能力受 4 细胞阶段细胞关联的影响”，《生殖生物学》53. 78-83 (1995)。

Tamai Y 他: "Mouse Elk oncogene maps on chromosome X and novel type Ets oncogene(Elk-3)maps on chromosome 10" Genomics. 26. 414-416 (1995)

Tamai Y 等人：“小鼠麋鹿癌基因位于 X 染色体上，新型 Ets 癌基因 (Elk-3) 位于 10 号染色体上”基因组学。

Iwakura Y,Saijo S 他: "Augmemtation of c-fos and c-jun expression in tumors in transgenic mice carrying the human T-cell leukemia virus type 1 tax gene." Virus Gene. (in press). (1994)

Iwakura Y、Saijo S 等人：“携带人类 T 细胞白血病病毒 1 型分类基因的转基因小鼠肿瘤中 c-fos 和 c-jun 表达的增强”（正在出版）。