Animal Experimental Model of Human Hepatocellular Carcinoma

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Basical problems about conditions of care and breeding woodchucks such as cages, housing or food were solved. We made stainless steel indoor cages(O. 36cm^2 floor space) which are very useful for keeping animals to observe them or make experiment but in this cage they did not mate or breed. In larger indoor iron boxes(2 m^2) animals could breed but new born pups were often killed by their dams. In our conclusion the best circumstances for breeding is outdoor permanent above ground cages in which litter size same as those of wild animals were obtained. Food pellets we produced are same as those of guinea pigs containing less crude fibers.We succeeded in establishing a method for obtaining artificial virus carriers by injecting virus into non virus carrier pupps. Even in 6 weeks old pupps we could get positive results. We conclude 2-4 weeks old pupps are best to be injected with virus as they are very active with open eyes and mortality is already low. Newly obtained carriers do not still develop cancers. We have checked positivity of hepatitis virus by RIA for surface antigen but we developed new technique utilizing polymerase chain reaction. As primer we used DNA part coding surface antigen.We followed wild spontaneous carriers for detecting hepatic cnacers and found when volume of cancer become larger than 10cm^3 they develop very rapidly. Integration pattern of virus DNA and n-myc rearrangement suggested that cancer develop both multicentric and metastatic. C-myc rearrangement occur very rare.