Investigating the evolutionary relationships of Arcellinida by single-cell phylogenomics of Phryganella (Arcellinida, Amoebozoa).

单细胞转录组学是建立健全的原生生物系统发育的新前沿技术。由此产生的大量数据使我们能够直接了解微生物的功能多样性。在变形虫中，形态多样的有壳变形虫基于形态学和拼凑的单基因系统发育分析被统一在阿米巴目下。甲壳类动物的形态特征在历史上具有重要的分类学和生态学意义，目前在生态学和古生态学研究中常被用作生物指标。然而，多基因方法揭示了传统的基于形态的分类的严重不一致性，并且最近显示了Arcellinida是多系的，挑战了任何基于传统的基于形态的分类的生态数据解释。为了进一步研究这一问题，我们对3个形态种的5个培养菌株的SSU rDNA序列进行了初步分析，结果表明，phryganellia科属群以Arcellinida为基础。我们得出的结论是，需要多基因的方法来澄清在阿米巴原虫中的系统发育位置。因此，单细胞转录组学将导致三种培养形态物种的彻底分子特征，进一步了解arcellinid testate变形虫的进化，学习这种方法将有助于个人的职业发展，并为我和同事未来的建议和研究奠定基础。

Single cell transcriptomics is the new cutting edge technique to establish sound protist phylogenies. The wealth of data thus generated enables a direct link to the functional diversity of microbes. In Amoebozoa, morphologically diverse shelled amoebae were unified under the order Arcellinida based on morphology and a patchwork of single-gene phylogenetic analyses. The characteristic and informative morphological characters present in the shell of the Arcellinida have been historically useful in taxonomy and ecology, nowadays they are often used as bioindicators in ecological and paleoecological studies. However, multigene approaches reveal severe inconsistencies in the traditional morphology-based classification and have recently shown the Arcellinida to be polyphyletic, challenging any ecological data interpretation based on traditional, morphology-based classification. To further investigate this, we obtained preliminary results from SSU rDNA sequences of five cultured strains from three morphospecies of the genus Phryganella, indicating that the family Phryganellidae groups at the basis of the Arcellinida. We came to the conclusion that multigene approaches are needed to clarify the phylogenetic position of Phryganella within the Amoebozoa. Single cell transcriptomes will thus lead to a thorough molecular characterization of the three cultured morphospecies, give further insight in the evolution of arcellinid testate amoebae and learning this method will facilitate personal career development and build the fundament for future proposals and research of myself and colleagues.