The effect of expression of Yamanaka factors on CHO cells

将四种因子（Yamanaka factors）导入体细胞，可以构建诱导多能干细胞（iPS）。在这项研究中，我们将这些因素引入中国仓鼠卵巢（CHO）细胞，这是生物制药生产的主力，并调查这些CHO细胞的特性。通过引入小鼠Oct 3/4、Klf 4和Sox 2因子，我们构建了过表达这些因子的CHO细胞。所构建的细胞的比生长速率和形态不受引入因子的影响。

Introduction of four factors, which are called Yamanaka factors, could construct induced pluripotential stem (iPS) cells from somatic cells. In this study, we introduce these factors into Chinese hamster ovary (CHO) cells which are workhorse for biopharmaceutical production and investigated the characteristics of these CHO cells. By introducing of mouse Oct3/4, Klf4, and Sox2 factors, we constructed CHO cells which over -expressed these factors. The specific growth rate and morphology of constructed cells were not affected by introduced factors.