The effect of expression of Yamanaka factors on CHO cells

引入四种被称为山中因子的因子，可以从体细胞构建诱导多能干细胞（iPS）。在本研究中，我们将这些因子引入到中国仓鼠卵巢细胞（CHO）中，并研究了这些细胞的特性。通过引入小鼠Oct3/4、Klf4和Sox2因子，构建了过表达这些因子的CHO细胞。所构建细胞的特定生长速率和形态不受引入因子的影响。

Introduction of four factors, which are called Yamanaka factors, could construct induced pluripotential stem (iPS) cells from somatic cells. In this study, we introduce these factors into Chinese hamster ovary (CHO) cells which are workhorse for biopharmaceutical production and investigated the characteristics of these CHO cells. By introducing of mouse Oct3/4, Klf4, and Sox2 factors, we constructed CHO cells which over -expressed these factors. The specific growth rate and morphology of constructed cells were not affected by introduced factors.