Rapid construction for gene-amplified cell line by chromosome targeting method

染色体打靶法快速构建基因扩增细胞系

• 批准号: 14550770
• 负责人: OMASA Takeshi
• 金额: $ 2.56万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14550770/
• 关键词: biologicals; Chinese hamster ovary cell; dihydrofolate reductase; methotrexate; chromosome targeting; gene amplification; gene amplification; dhfr; CHO; methotrexate; MTX

Biologicals are important pharmaceutical products and recently the production is growing up. The most conventional method for biologicals production is using mammalian cell culture process. The most important points for production of biologicals using mammalian cell culture is the construction of mammalian cell line for production. Gene amplification is most conventional and complicated process for construction of productive mammalian cell. In this research, we investigated various types of MTX increasing methods using image analysis and obtained suitable gene amplification location of chromosome "P". We constructed suitable host cell line for gene amplification in location "P" and the objective gene was inserted in this location by recombinase reaction.

生物制品是重要的医药产品，近年来其生产规模不断扩大。最传统的生物制品生产方法是利用哺乳动物细胞培养过程。利用哺乳动物细胞培养生产生物制品，最重要的是构建用于生产的哺乳动物细胞系。基因扩增是构建高产哺乳动物细胞最常规和最复杂的过程。在本研究中，我们用图像分析的方法研究了不同类型的MTX增加方法，得到了染色体“P”的合适基因扩增位置。我们构建了适合于基因扩增的宿主细胞系“P”，并通过重组酶反应将目的基因插入到该位置。

项目成果

Construction of liver model with genetically engineered human HepG2 cells

基因工程人HepG2细胞构建肝脏模型

• 发表时间: 2004
• 期刊: Animal Cell Technology : Basic & Applied Aspects 13
• 作者: Takeshi Omasa;Shin Enosawa
• 通讯作者: Shin Enosawa

Expression and amplification of glutamine synthetase gene for constructing ammonia-metabolizing cell lines in hybrid bioartificial liver support system

混合生物人工肝支持系统中谷氨酰胺合成酶基因的表达和扩增构建氨代谢细胞系

• 发表时间: 2002
• 期刊: Animal Cell Technology : Basic & Applied Aspects 12
• 作者: Takeshi Omasa;Kazumi Kim;Shin-ya Hiramastu;Yoshio Katakura;Michimasa Kishimoto;Toshiharu Mastumura;Shin Enosawa;Seiichi Suzuki;hiroshi Amemiya;Ken-ichi Suga
• 通讯作者: Ken-ichi Suga

Frontier of antibody engineering (ISBN4-88231-825-3)

抗体工程前沿（ISBN4-88231-825-3）

• 发表时间: 2004
• 作者: Takeshi Omasa;Kazumi Kim;Shin-ya Hiramatsu;Yoshio Katakura;Michimasa Kishimoto;Toshiharu Matsumura;Shin Enosawa;Seiichi Suzuki;Hiroshi Amemiya;Ken-ichi Suga;Takeshi Omasa;大政健史;Takeshi Omasa
• 通讯作者: Takeshi Omasa

Flow cytometly : Rapid construction of gene-amplified CHO cell line by gene targeting

流式细胞仪：通过基因打靶快速构建基因扩增的 CHO 细胞系

• 发表时间: 2004
• 期刊: Proc.for the 10th APCChe 2004
• 作者: Takeshi Omasa;Mihoko Kajita;Tomohiro;Yoshikawa;Yoshio Katakura;Michimasa Kishimoto;Hisao Ohtake
• 通讯作者: Hisao Ohtake

抗体エンジニアリングの最前線

抗体工程前沿

• 发表时间: 2004
• 作者: 植田 充美(監修);上田 宏(共著)
• 通讯作者: 上田 宏(共著)