Rapid construction for gene-amplified cell line by chromosome targeting method

生物制品是重要的医药产品，近年来产量不断增长。最传统的生物制剂生产方法是利用哺乳动物细胞培养过程。利用哺乳动物细胞培养生产生物制品最重要的一点是哺乳动物细胞系的构建。基因扩增是构建哺乳动物细胞最常规、最复杂的方法。在本研究中，我们利用图像分析的方法研究了不同类型的MTX增加方法，获得了“P”染色体合适的基因扩增位置。我们在“P”位置构建了适合基因扩增的宿主细胞系，并通过重组酶反应将目的基因插入该位置。

Biologicals are important pharmaceutical products and recently the production is growing up. The most conventional method for biologicals production is using mammalian cell culture process. The most important points for production of biologicals using mammalian cell culture is the construction of mammalian cell line for production. Gene amplification is most conventional and complicated process for construction of productive mammalian cell. In this research, we investigated various types of MTX increasing methods using image analysis and obtained suitable gene amplification location of chromosome "P". We constructed suitable host cell line for gene amplification in location "P" and the objective gene was inserted in this location by recombinase reaction.