Genome-wide analysis and its application in Chinese hamster ovary cells

中国仓鼠卵巢(CHO)细胞是工业化生产治疗性蛋白最可靠的宿主细胞。我们从基因扩增的CHO细胞系中构建了CHO基因组细菌人工染色体(BAC)文库，用于CHO细胞系的全基因组分析。以BAC克隆为杂交探针，通过荧光原位杂交(FISH)技术构建CHO染色体标记。使用构建的工具，我们可以分析CHO细胞系的染色体重排。

Chinese hamster ovary (CHO) cells are the most dependable host cells for the industrial production of therapeutic proteins. We constructed CHO genomic bacterial artificial chromosome (BAC) library from a gene-amplified CHO cell line for genome-wide analysis of CHO cell lines. CHO chromosomal markers were constructed by fluorescence in situ hybridization (FISH) imaging using BAC clones as hybridization probes (BAC-FISH). Using constructed tools, we could analyze chromosome rearrangement in CHO cell line.