Affinity Maturation of Antibodies by Induction of Gene Conversion or Somatic Hypermutation.

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In adaptive immune system, somatic hyper mutation in hyper-variable regions of antibody loci leads to affinity maturation of antibodies that strengthens the antigen binding activities. We previously developed an in vitro-based antibody design system using chicken B cell line DT40. This system, referred to as “ADLib (Autonomously Diversifying Library) system”, allows very rapid monoclonal antibodies production (within a week). In this study, ADLib system is newly equipped with the somatic hypermutation process, which is regulated by AID (auxin-inducible degron). In other words, affinity maturation is reconstituted in vitro by the use of DT40. Using this system, we can improve affinity of antibodies substantially.