Development of electron microscopy atlas: Fusion with omics and electron microscopic data

电子显微镜图谱的开发：组学和电子显微镜数据的融合

• 批准号: 23657051
• 负责人: TOYOOKA Kiminori
• 金额: $ 2.5万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23657051/
• 关键词: 電子顕微鏡; 高圧凍結技法; 電子地図; オルガネラ; 超微形態

Transmission electron microscopy (TEM) is important for high-resolution ultrastructure observation for organelles, cell and tissues. However, it is unsuitable for comprehensive analyses. I constructed TEM atlas to achieve an exhaustive ultrastructural analysis of organelles in model plants by using high-pressure freezing (HFP) and TEM techniques. To take wide and high-resolution TEM pictures, Arabidopsis roots and BY2 cells were frozen by HFP machine and then fix. After embedded in resin, large ultrathin sections were put on a hole grid. Then we took TEM pictures by auto-digital TEM recoding system. I succeed in records more than 30 thousand images. We developed auto-photomerge program for TEM pictures and several thousands of pictures were automatically merged. Using these wide-high resolution pictures, I have developed a web-based 2D map that can be zoomed and searched.

透射电子显微镜（TEM）是高分辨率观察细胞器、细胞和组织超微结构的重要手段。但是，它不适合进行全面的分析。我构建了TEM图谱，通过使用高压冷冻（HFP）和TEM技术对模式植物中的细胞器进行详尽的超微结构分析。为了拍摄宽范围、高分辨率的透射电镜照片，将拟南芥根和BY2细胞用HFP冷冻机冷冻后固定。在树脂包埋后，将大的切片放在孔网格上。然后用自动数字TEM记录系统拍摄TEM照片。我成功地记录了3万多张照片。研制了TEM照片自动拼接程序，实现了数千张TEM照片的自动拼接。利用这些宽高分辨率的图片，我开发了一个基于网络的2D地图，可以缩放和搜索。

项目成果

生体顕微マルチスケールマッピングシステム:小胞クラスターの分布と構造

生物微观多尺度绘图系统：囊泡簇的分布和结构

• 发表时间: 2012
• 作者: 豊岡公徳;若崎眞由美;朽名夏麿;桧垣匠;永田典子;松岡健;持田恵一;佐藤繭子
• 通讯作者: 佐藤繭子

Immunohistochemical observation of indole-3-acetic acid at the IAA synthetic maize coleoptile tips

• DOI: 10.4161/psb.6.12.18080
• 发表时间: 2011-12
• 期刊: Plant Signaling & Behavior
• 影响因子: 2.9
• 作者: Takeshi Nishimura;K. Toyooka;Mayuko Sato;S. Matsumoto;M. M. Lucas-M.;M. Strnad;F. Baluška;T. Koshiba

小胞輸送系での物質合成・輸送関連タンパク質と脂質膜ドメイン

囊泡运输系统中的材料合成/运输相关蛋白和脂膜结构域

• 发表时间: 2012
• 作者: Inaba;J.;Hidema J.;松岡健,豊岡公徳
• 通讯作者: 松岡健,豊岡公徳

Micro-CT observations of the 3D distribution of calcium oxalate crystals in cotyledons during maturation and germination in Lotus miyakojimae seeds.

• DOI: 10.1093/jmicro/dfs079
• 发表时间: 2013-06
• 期刊: Microscopy
• 影响因子: 1.8
• 作者: D. Yamauchi;Daisuke Tamaoki;Masato Hayami;M. Takeuchi;I. Karahara;Mayuko Sato;K. Toyooka;H. Nishioka;Y. Terada;K. Uesugi;H. Takano;Y. Kagoshima;Y. Mineyuki

Plasma Membrane Aquaporin AqpZ Protein Is Essential for Glucose Metabolism during Photomixotrophic Growth of Synechocystis sp PCC 6803

• DOI: 10.1074/jbc.m111.236380
• 发表时间: 2011-07-15
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Akai, Masaro;Onai, Kiyoshi;Uozumi, Nobuyuki
• 通讯作者: Uozumi, Nobuyuki