Role of circadian regulator BMAL1 in chromatin remodeling

染色质是真核生物基因组组成的核蛋白结构，它使转录调控等重要生物过程得以实现。各种重塑事件使染色质的结构在浓缩和去浓缩状态之间转换，每种状态都与特定的细胞功能相耦合。共价修饰发生在核心组蛋白H2A、H2B、H3和H4的n端尾部。一些组蛋白修饰有助于染色质重塑，从而控制大量的核过程。此外，尾部的位置使得这些区域可以进行修饰，从而可逆地调节染色质结构。虽然死亡结构域相关蛋白DAXX传统上被认为与细胞死亡途径的调控有关，但最近的研究表明DAXX可以作为组蛋白伴侣发挥作用。生物钟是内在的时间跟踪系统，赋予生物体生存优势。在哺乳动物中，转录因子BMAL1是必不可少的昼夜节律调节因子。我已经确定BMAL1是一种新的daxx相互作用蛋白。这项研究提供了几条证据，表明DAXX-BMAL1相互作用可能在染色质转变的动态变化中起重要作用。

Chromatin, the nucleoprotein structure into which the eukaryotic genome is organized, enables essential biological processes such as regulation of transcription. A variety of remodeling events enable the architecture of chromatin to transition between a condensed and a decondensed state, each state being coupled to specific cellular functions. The covalent modifications occur on the N-terminal tails of the core histones H2A, H2B, H3 and H4. Several histone modifications contribute to chromatin remodeling and thereby to the control of a large array of nuclear processes. In addition, the disposition of the tails renders these domains accessible to modifications that could reversibly modulate chromatin structure. Although the death domain-associated protein DAXX has traditionally been associated with the regulation of cell death pathways, recent studies have demonstrated that DAXX can function as a histone chaperone.Circadian clocks are intrinsic, time-tracking systems that endow organisms with a survival advantage. In mammals, the transcription factors, BMAL1, is an essential circadian regulator. I have identified BMAL1 as a novel DAXX-interacting protein. This study provides several lines of evidence indicating that DAXX-BMAL1 interaction may have important roles in dynamic changes in chromatin transitions.