The Influences of Laser Irradiation on Cells

本研究的目的是测量激光照射后不同类型细胞内的活性氧(ROS)生成。ROS被认为是低强度激光治疗(LLLT)产生的关键次级信使。尽管LLLT的作用机制多种多样，细胞内ROS被认为是影响LLLT的关键因素之一，但在不同波长的激光照射下，几种细胞内ROS的直接测量尚未见报道。相反，红光激光或近红外激光照射后，未观察到细胞内ROS的产生。蓝光照射可激发细胞内潜在的ROS源，导致细胞内ROS的产生。

The purpose of this study was to measure intracellular reactive oxygen species (ROS) production after laser irradiation in various types of cells. ROS are considered to be the key secondary messengers produced by low-level laser therapy (LLLT). Although various mechanisms for the effects of LLLT have been proposed, and intracellular ROS were indicated as the one of the key factors, direct measurement of intracellular ROS of several types of cells after different wavelength lasers irradiation has not been reported.After a blue laser irradiation, intracellular ROS levels were increased in all types of cells. In contrast, intracellular ROS generation was not observed after irradiation with a red laser or near-infrared laser. Potential sources of intracellular ROS were excited by blue laser irradiation, resulting in ROS production within cells.