The Influences of Laser Irradiation on Cells

激光照射对细胞的影响

• 批准号: 23657102
• 负责人: KUSHIBIKI Toshihiro
• 金额: $ 2.41万
• 依托单位: National Defense Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23657102/
• 关键词: レーザー; 細胞; 機能制御; 光

The purpose of this study was to measure intracellular reactive oxygen species (ROS) production after laser irradiation in various types of cells. ROS are considered to be the key secondary messengers produced by low-level laser therapy (LLLT). Although various mechanisms for the effects of LLLT have been proposed, and intracellular ROS were indicated as the one of the key factors, direct measurement of intracellular ROS of several types of cells after different wavelength lasers irradiation has not been reported.After a blue laser irradiation, intracellular ROS levels were increased in all types of cells. In contrast, intracellular ROS generation was not observed after irradiation with a red laser or near-infrared laser. Potential sources of intracellular ROS were excited by blue laser irradiation, resulting in ROS production within cells.

本研究的目的是测量激光照射后不同类型细胞内的活性氧(ROS)生成。ROS被认为是低强度激光治疗(LLLT)产生的关键次级信使。尽管LLLT的作用机制多种多样，细胞内ROS被认为是影响LLLT的关键因素之一，但在不同波长的激光照射下，几种细胞内ROS的直接测量尚未见报道。相反，红光激光或近红外激光照射后，未观察到细胞内ROS的产生。蓝光照射可激发细胞内潜在的ROS源，导致细胞内ROS的产生。

项目成果

Responses of cancer cells induced by photodynamic therapy.

• DOI: 10.1260/2040-2295.4.1.87
• 发表时间: 2013-03
• 期刊: Journal of healthcare engineering
• 作者: T. Kushibiki;T. Hirasawa;S. Okawa;M. Ishihara

Immunological aspects of photodynamic therapy

光动力疗法的免疫学方面

• 发表时间: 2013
• 期刊: Trends Photochem Photobiol
• 作者: Kushibiki T;Hirasawa T;Okawa S;Ishihara M
• 通讯作者: Ishihara M

Blue Laser Irradiation Generates Intracellular Reactive Oxygen Species in Various Types of Cells

• DOI: 10.1089/pho.2012.3361
• 发表时间: 2013-03-01
• 期刊: PHOTOMEDICINE AND LASER SURGERY
• 作者: Kushibiki, Toshihiro;Hirasawa, Takeshi;Ishihara, Miya
• 通讯作者: Ishihara, Miya