Functional analysis of an insect sex determining gene found inSaccoglossus kowalevskii genome.

对 Saccoglossus kowalevskii 基因组中发现的昆虫性别决定基因的功能分析。

• 批准号: 23657154
• 负责人: SUZUKI Masataka
• 金额: $ 2.5万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23657154/
• 关键词: 性決定; 性分化; 選択的スプライシング; ギボシムシ; 進化; 性決定遺伝子; ミジンコ; 国際情報交流; transformer遺伝子; 半索動物

We have found a gene (here after, depicted as Sktra) in Saccoglossus kowalevskii genome that is closely similar to a tra gene which is known to act as a central role in insect sex determination and determined its full-length cDNA sequence and its exon/intron structure. RT-PCR analyses demonstrated that, differ from the insect tra gene, no sex-specific splicing variants were observed in Sktra transcripts. The expression level of Sktra mRNA in testes is 9-fold higher than in ovaries. This result raises the possibility that Sktra is involved in sexual differentiation of S. kowalevskii. Splicing assays using insect culture cells revealed that Sktra can induce female-specific splicing of Drosophila dsx minigene pre-mRNA when co-transfected together with Sktra-2 cDNA. This result strongly suggests that the splicing activity specifically identified in insect Tra proteins is conserved in SkTra protein. We provide the world’s first evidence that demonstrates functional conservation of tra gene found in deuterostomes.

我们已经发现了一个基因（在这里，描述为Sktra）在囊舌象kowalevskii基因组中，是非常相似的tra基因，这是已知的作为一个中心作用，在昆虫性别决定，并确定其全长cDNA序列和外显子/内含子结构。RT-PCR分析表明，与昆虫tra基因不同，Sktra转录本中没有观察到性别特异性剪接变体。睾丸中Sktra mRNA的表达水平是卵巢中的9倍。这一结果提出了Sktra参与S.科瓦列夫斯基。昆虫培养细胞的剪接实验表明，Sktra与Sktra-2 cDNA共转染后，可诱导果蝇dsx小基因前体mRNA发生雌性特异性剪接。这一结果有力地表明，在昆虫Tra蛋白中特异性鉴定的剪接活性在SkTra蛋白中是保守的。我们提供了世界上第一个证明后口动物tra基因功能保守的证据。

项目成果

The Expression and Nuclear Deposition of Histone H3.1 in Murine Oocytes and Preimplantation Embryos

• DOI: 10.1262/jrd.2012-074
• 发表时间: 2012-10-01
• 期刊: JOURNAL OF REPRODUCTION AND DEVELOPMENT
• 影响因子: 1.8
• 作者: Kawamura, Machika;Akiyama, Tomohiko;Aoki, Fugaku
• 通讯作者: Aoki, Fugaku

カイコにおける遺伝的性決定機構

家蚕的遗传性别决定机制

• 发表时间: 2012
• 作者: Kawamura M;Akiyama T;Tsukamoto S;Suzuki MG;Aoki F.;Machika Kawamura;鈴木雅京
• 通讯作者: 鈴木雅京

なし

没有任何

• 发表时间: 2020
• 期刊: なし
• 作者: 佐竹 澄子;務台理恵子;高塚綾子;なし