Genome wide analysis for genes involved in the lepidopteran insect sex determination cascade and its application to pest control

鳞翅目昆虫性别决定级联相关基因的全基因组分析及其在害虫防治中的应用

• 批准号: 23380032
• 负责人: SUZUKI Masataka
• 金额: $ 10.23万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23380032/
• 关键词: 性決定; 性分化; 性決定遺伝子; 選択的スプライシング; de novo トランスクリプトーム; カイコ; 害虫防除; W染色体; 発生・分化; CRISPR/Cas9; ヒストン修飾; ヒストンH3メチル化; 自己スプライシング制御; transformer-2遺伝子; doublesex遺伝子

We found that a Bombyx homolog of tra-2 is dispensable for the sex-specific splicing of Bmdsx pre-mRNA. Instead, a male-specific splice variant of Imp is involved in the male-specific splicing of Bmdsx. The male-specific expression of Imp is controlled by its autoregulatory function, whereby the protein product regulates the male-specific splicing of its own pre-mRNA. This suggests that Imp acts as the memory device for sex determination in the silkworm. In an attempt to identify the Fem gene of B. mori, we performed comparative analysis of de novo transcriptome assembly between male and female embryos at the sex-determining stage. Among 149 cDNAs that were expressed at more than eight-fold higher in females than in males, two cDNAs (FET-W1, FET-W2) were derived from genes located on the sex-determining region of the W chromosome. Knockdown of FETW-1 induced expression of male-type Bmdsx and ImpM in female eggs. These results suggest that FET-W1 is the most likely candidate for Fem.

我们发现，Tra-2的Bombyx同源物对于BMDSX Pre-MRNA的性别特异性剪接是可分配的。取而代之的是，IMP的男性特异性剪接变体与BMDSX的男性特异性剪接有关。 IMP的男性特异性表达受其自动调节功能的控制，从而调节其自身前MRNA的男性特异性剪接。这表明，IMP充当蚕中性别确定的记忆装置。为了鉴定莫里氏芽孢杆菌的FEM基因，我们对男性和女性胚胎在性别确定的阶段进行了从头转录组的比较分析。在女性中比男性高八倍以上表达的CDNA中，两个cDNA（FET-W1，FET-W2）来自位于W染色体的性别确定区域上的基因。 FETW-1敲低雌性卵中雄性型BMDSX和IMPM的表达。这些结果表明，FET-W1是FEM的最可能候选者。

项目成果

CRISPR-Cas システムを用いたゲノム編集によるカイコ IMP ホモログ(IMP)の機能解析

使用 CRISPR-Cas 系统进行基因组编辑对家蚕 IMP 同源物 (IMP) 进行功能分析

• 发表时间: 2014
• 作者: 坂口穂菜美;酒井弘貴;青木不学;鈴木雅京
• 通讯作者: 鈴木雅京

カイコ transformer-2 ホモログ(Bmtra2)の機能解析

蚕transformer-2同源物(Bmtra2)的功能分析

• 发表时间: 2011
• 作者: 鈴木啓二;青木不学;鈴木雅京
• 通讯作者: 鈴木雅京

Involvement of DOT1L in the Remodeling of Heterochromatin Configuration During Early Preimplantation Development in Mice

• DOI: 10.1095/biolreprod.113.113258
• 发表时间: 2013-12-01
• 期刊: BIOLOGY OF REPRODUCTION
• 影响因子: 3.6
• 作者: Ooga, Masatoshi;Suzuki, Masataka G.;Aoki, Fugaku
• 通讯作者: Aoki, Fugaku

The Expression and Nuclear Deposition of Histone H3.1 in Murine Oocytes and Preimplantation Embryos

• DOI: 10.1262/jrd.2012-074
• 发表时间: 2012-10-01
• 期刊: JOURNAL OF REPRODUCTION AND DEVELOPMENT
• 影响因子: 1.8
• 作者: Kawamura, Machika;Akiyama, Tomohiko;Aoki, Fugaku
• 通讯作者: Aoki, Fugaku

Appearance of differentiated cells derived from polar body nuclei in the silkworm

家蚕极体核分化细胞的出现

• 发表时间: 2013
• 期刊: Bombyx mori. Frontiers in Physiology .
• 作者: Sakai H.;Yokoyama T.;Abe H.;Fujii T.;Suzuki M. G
• 通讯作者: Suzuki M. G