Development of an innovative method for reducing colon-cancer-inducing secondary bile acid formation through enhancement of anaerobic respiration of the intestinal bacteria

开发一种通过增强肠道细菌无氧呼吸来减少结肠癌诱导的次级胆汁酸形成的创新方法

• 批准号: 23658064
• 负责人: YOKOTA Atsushi
• 金额: $ 2.41万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23658064/
• 关键词: 二次胆汁酸; デオキシコール酸; リトコール酸; フマル酸; 嫌気呼吸; 腸内細菌; 大腸癌; 腸溶性カプセル

Some secondary bile acids formed in the large intestine are known as a riskfactor of colon cancer. In this study a novel method that reduces such secondary bile acids formed by thereductive metabolism of gut microbes were investigated. We tested if the enhancement of oxidativemetabolism of the responsible gut microbes by the introduction of anaerobic respiration can reduce thetarget secondary bile acids. Thus, rats were fed sodium fumarate-supplemented diet to enhanceanaerobic respiration of the gut microbes. Although the results suggested a possibility of decreasingrelative abundance of deoxycholic acid, a representative colon-cancer-inducing secondary bile acid, theobserved effects were not practically applicable, because high dose of fumarate was required (5~10% ofthe diet), which resulted in the diarrhea in rats experiments. Therefore, improved method of fumaratefeeding, e.g. encapsulation, might be necessary to achieve this goal.

一些在大肠中形成的次级胆汁酸被认为是结肠癌的危险因素。在这项研究中，一种新的方法，减少这种二级胆汁酸形成的还原代谢的肠道微生物进行了调查。我们测试了通过引入无氧呼吸来增强负责肠道微生物的氧化代谢是否可以减少目标次级胆汁酸。因此，给大鼠喂食补充丙酸钠的饮食以增强肠道微生物的无氧呼吸。虽然实验结果表明，富马酸可能降低了诱导结肠癌的代表性次级胆汁酸脱氧胆酸的相对丰度，但观察到的效果并不实际适用，因为富马酸需要高剂量（饲料的5~10%），这导致了大鼠实验中的腹泻。因此，可能需要改进的浓缩物进料方法，例如封装，以实现这一目标。

项目成果

胆汁酸と腸内フローラ

胆汁酸和肠道菌群

• 发表时间: 2012
• 作者: Atsushi Yokota;横田篤
• 通讯作者: 横田篤

Is bile acid a determinant of the gut microbiota on a high-fat diet?

• DOI: 10.4161/gmic.21216
• 发表时间: 2012-07
• 期刊: Gut Microbes
• 影响因子: 12.2
• 作者: A. Yokota;S. Fukiya;K. Islam;Tadasuke Ooka;Yoshitoshi Ogura;Tetsuya Hayashi;Masahito Hagio;S. Ishizuka-S.

Bile Acid Is a Host Factor That Regulates the Composition of the Cecal Microbiota in Rats

• DOI: 10.1053/j.gastro.2011.07.046
• 发表时间: 2011-11-01
• 期刊: GASTROENTEROLOGY
• 影响因子: 29.4
• 作者: Islam, K. B. M. Saiful;Fukiya, Satoru;Yokota, Atsushi
• 通讯作者: Yokota, Atsushi

新規ウルソデオキシコール酸生成腸内細菌Ruminococcus gnavus N53 由来の 7 β-hydroxysteroid dehydrogenaseの発現と機能解析

新型产熊去氧胆酸肠杆菌 gnavus N53 7β-羟基类固醇脱氢酶的表达和功能分析

• 发表时间: 2012
• 作者: K.B.M. S. Islam;S. Fukiya;M. Hagio;N.Fujii;S. Ishizuka;T. Ooka;Y. Ogura;T.Hayashi;A. Yokota;李慈英,吹谷智,和田大,横田篤
• 通讯作者: 李慈英,吹谷智,和田大,横田篤

新規ウルソデオキシコール酸生成腸内細菌 Ruminococcus gnavus N53由来の 7β-hydroxysteroid dehydrogenaseの発現と機能解析

一种新型产熊去氧胆酸肠道细菌疣状瘤胃球菌 N53 来源的 7β-羟基类固醇脱氢酶的表达和功能分析

• 发表时间: 2012
• 作者: K.B.M. S. Islam;S. Fukiya;M. Hagio;N.Fujii;S. Ishizuka;T. Ooka;Y. Ogura;T.Hayashi;A. Yokota;李慈英,吹谷智,和田大,横田篤;李 慈英,荒井尚志,吹谷智,和田大,横田篤
• 通讯作者: 李 慈英,荒井尚志,吹谷智,和田大,横田篤