Metagenomics using porous arrowhead devices; from environmental assessment to screening

使用多孔箭头装置的宏基因组学；

• 批准号: 23658067
• 负责人: NAKAJIMA Toshiaki
• 金额: $ 2.5万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23658067/
• 关键词: 環境微生物; メタゲノム; 微生物スクリーニング

As a tool of environmental metagenomics, an arrowhead device made of porous material was suggested. Impacts against microbial flora of various chemical compounds were investigated by using this device. In addition, accumulation of microorganisms with specific function is tried by infiltrating a screening object into this device. As a result, I succeeded to produce specific microbial flora to the outskirts of this device by gradually releasing a target material from it. Furthermore, around 5cm was enough for the distance of the arrowhead to get good result. Thus this method is space-saving. This method must be a cheap, and simple metagenome technology in future.

作为环境宏基因组学研究的一种工具，提出了一种由多孔材料制成的箭头装置。使用该装置研究了各种化合物对微生物植物群的影响。此外，通过将筛选对象渗透到该装置中来尝试具有特定功能的微生物的积累。结果，通过逐渐释放目标物质，我成功地在该装置的外围产生了特定的微生物植物群。此外，大约5 cm的箭头距离足以获得良好的结果。因此，这种方法是节省空间的。该方法是一种廉价、简便的宏基因组技术。