Metagenomics using porous arrowhead devices; from environmental assessment to screening

使用多孔箭头装置的宏基因组学；

• 批准号: 23658067
• 负责人: NAKAJIMA Toshiaki
• 金额: $ 2.5万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23658067/
• 关键词: 環境微生物; メタゲノム; 微生物スクリーニング

As a tool of environmental metagenomics, an arrowhead device made of porous material was suggested. Impacts against microbial flora of various chemical compounds were investigated by using this device. In addition, accumulation of microorganisms with specific function is tried by infiltrating a screening object into this device. As a result, I succeeded to produce specific microbial flora to the outskirts of this device by gradually releasing a target material from it. Furthermore, around 5cm was enough for the distance of the arrowhead to get good result. Thus this method is space-saving. This method must be a cheap, and simple metagenome technology in future.

作为环境宏基因组学的一种工具，提出了一种由多孔材料制成的箭头装置。利用该装置研究了不同化合物对微生物区系的影响。此外，通过将筛选对象渗透到该装置中，尝试积累具有特定功能的微生物。结果，我成功地在这个设备的外围产生了特定的微生物菌群，逐渐从它释放出一种目标材料。此外，箭头的距离在5厘米左右即可获得较好的效果。因此，这种方法节省了空间。这种方法在未来一定是一种廉价、简单的元基因组技术。