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Function and regulatory mechonismes of ionic chapnels in vasculer snoock muscle cells University of Tokyo

血管斯诺克肌细胞离子通道的功能和调节机制东京大学

基本信息

批准号：
07670760
负责人：
NAKAJIMA Toshiaki
金额：
$ 1.28万
依托单位：
UNIVERSITY of TOKYO
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670760/
关键词：
vascular smooth muscle ionic currents vasopressin esdothelin-1 non-selective cation channel Mg^<2+>fatty a cids Ca^<2+> activated Cl^- current vascular smooth muscle vasopressin non-seledue cation channel Ca^<2+>-activated Cl^- curr

项目摘要

1) To clarify the significance and characterization of Ca^<2+> -permeable nonselective cation channels activated by vasopressin and endothelin-1 (receptor-mediated non-selective cation channels) in vascular smooth muscle cells, the patch clamp techniques, Ca^<2+> or tension measurement were applied in aortic smooth muscle cells. The results suggested that calcium ectry and membrane depolarization elicited by these agonists are partly mediated by a receptormediated Ca^<2+> -permeable non-selective cation channel in vascular smooth muscle cells. Also, we showed the characteristics and activation mechanisms of the channel. Furthermore, we investigated the effects of extracellular Mg^<2+> and omega-3 polyunsaturated fatty acids, which are known to produce vascular relaxation or hypotensive effects, on the receptor-mediated non-selective cation channels. We showed that extracellular Mg^<2+> and omega-3 polyunsaturated fatty acids effectively inhibit receptor-mediated non-selective cation channel. which may play an important role in the regulation of vascular tone.2) In tracheal myocytes, to investigate the activation mechanism and characterization of Ca^<2+> -dependent Cl^- current, the patch clamp technique was applied. We presented that Ca^<2+> -dependent Cl^- current can be activated by SR (sarcoplasmic reticulum) Ca^<2+> release due to neurokinin A or caffeine (through IP_3 or ryanodine receptors) as well as by Ca^<2+> infulx due to the voltage-dependent L-type Ca^<2+> current. It also showed that the functional coupling of ryanodine receptor to the voltage-dependent L-type Ca^<2+> channels exists in tracheal smooth muscle cells.

1)为了阐明血管加压素和内皮素-1(受体介导的非选择性阳离子通道)激活的钙通透性非选择性阳离子通道的意义和特性，采用膜片钳技术、钙离子非选择性阳离子通道和张力测定技术对血管平滑肌细胞进行了研究。结果提示，这些激动剂引起的钙通道和膜去极化作用部分是由受体介导的非选择性阳离子通道介导的。此外，我们还展示了该通道的特征和激活机制。此外，我们还研究了细胞外镁离子和omega-3多不饱和脂肪酸对受体介导的非选择性阳离子通道的影响。我们发现胞外镁离子和omega-3多不饱和脂肪酸能有效地抑制受体介导的非选择性阳离子通道。2)在气管肌细胞上，应用膜片钳技术研究依赖钙离子的氯离子电流的激活机制和特性。我们提出，肌浆网钙离子电流既可被由神经激动素A或咖啡因(通过IP_3或ryanodine受体)释放引起的肌浆网钙离子电流激活，也可由电压依赖性L型钙离子电流引起的钙离子内流激活。研究还表明，在气管平滑肌细胞中，兰尼定受体与电压依赖性的L型钙通道存在功能性偶联。