Function and regulatory mechonismes of ionic chapnels in vasculer snoock muscle cells University of Tokyo

血管斯诺克肌细胞离子通道的功能和调节机制东京大学

基本信息

  • 批准号:
    07670760
  • 负责人:
  • 金额:
    $ 1.28万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    1995
  • 资助国家:
    日本
  • 起止时间:
    1995 至 1996
  • 项目状态:
    已结题

项目摘要

1) To clarify the significance and characterization of Ca^<2+> -permeable nonselective cation channels activated by vasopressin and endothelin-1 (receptor-mediated non-selective cation channels) in vascular smooth muscle cells, the patch clamp techniques, Ca^<2+> or tension measurement were applied in aortic smooth muscle cells. The results suggested that calcium ectry and membrane depolarization elicited by these agonists are partly mediated by a receptormediated Ca^<2+> -permeable non-selective cation channel in vascular smooth muscle cells. Also, we showed the characteristics and activation mechanisms of the channel. Furthermore, we investigated the effects of extracellular Mg^<2+> and omega-3 polyunsaturated fatty acids, which are known to produce vascular relaxation or hypotensive effects, on the receptor-mediated non-selective cation channels. We showed that extracellular Mg^<2+> and omega-3 polyunsaturated fatty acids effectively inhibit receptor-mediated non-selective cation channel. which may play an important role in the regulation of vascular tone.2) In tracheal myocytes, to investigate the activation mechanism and characterization of Ca^<2+> -dependent Cl^- current, the patch clamp technique was applied. We presented that Ca^<2+> -dependent Cl^- current can be activated by SR (sarcoplasmic reticulum) Ca^<2+> release due to neurokinin A or caffeine (through IP_3 or ryanodine receptors) as well as by Ca^<2+> infulx due to the voltage-dependent L-type Ca^<2+> current. It also showed that the functional coupling of ryanodine receptor to the voltage-dependent L-type Ca^<2+> channels exists in tracheal smooth muscle cells.
1)为了阐明血管加压素和内皮素-1(受体介导的非选择性阳离子通道)激活的钙通透性非选择性阳离子通道的意义和特性,采用膜片钳技术、钙离子非选择性阳离子通道和张力测定技术对血管平滑肌细胞进行了研究。结果提示,这些激动剂引起的钙通道和膜去极化作用部分是由受体介导的非选择性阳离子通道介导的。此外,我们还展示了该通道的特征和激活机制。此外,我们还研究了细胞外镁离子和omega-3多不饱和脂肪酸对受体介导的非选择性阳离子通道的影响。我们发现胞外镁离子和omega-3多不饱和脂肪酸能有效地抑制受体介导的非选择性阳离子通道。2)在气管肌细胞上,应用膜片钳技术研究依赖钙离子的氯离子电流的激活机制和特性。我们提出,肌浆网钙离子电流既可被由神经激动素A或咖啡因(通过IP_3或ryanodine受体)释放引起的肌浆网钙离子电流激活,也可由电压依赖性L型钙离子电流引起的钙离子内流激活。研究还表明,在气管平滑肌细胞中,兰尼定受体与电压依赖性的L型钙通道存在功能性偶联。

项目成果

期刊论文数量(24)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
H.Hazama et al.: "Neurokinis A and Ca^<2+> current induce Ca^<2+>-activated Cl^- currents in guinea-peg tracheal Nyooytes" Journal of Physiology (London). 492. 377-393 (1996)
H.Hazama等人:“Neurokinis A和Ca^2电流在几内亚钉气管Nyooytes中诱导Ca^2-激活的Cl^-电流”生理学杂志(伦敦)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
T.Nakajima, K.Iwasawa, H.Hazama, M.Asano, Y.Okuda, M.Omata: "Extracellular Mg^<2+> inhibits receptor-mediated Ca^<2+>-permeable non-selective cation currents in aortic smooth muscle cells." European Journal of Pharmacology. (in press). (1997)
T.Nakajima、K.Iwasawa、H.Hazama、M.Asano、Y.Okuda、M.Omata:“细胞外 Mg^2 > 抑制主动脉平滑肌中受体介导的 Ca^2 > 渗透性非选择性阳离子电流
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
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  • 通讯作者:
E.Hamada et al.: "Effect of caffeine on mucus secretion and agonist-dependent Ca^<2+> mobilization in human gastric mucus-secreting cells" Biochem.Biophys ACTA. (in press). (1997)
E.Hamada等人:“咖啡因对人胃粘液分泌细胞中粘液分泌和激动剂依赖性Ca 2+ 动员的影响”Biochem.Biophys ACTA。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
E.Hamada et al.: "Effects of caffeien on mucus secretion and agonist dеpendent Ca^<2+> mobilization in human gastus muscle secreting cells" Biochem.,Biophys ACTA. (in press). (1997)
E. Hamada 等人:“咖啡因对人胃肌分泌细胞中粘液分泌和激动剂依赖性 Ca^2+ 动员的影响”Biochem.,Biophys ACTA(出版中)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
T.Nakajima, H.Hazama, E.Hamada, S.H.Wu, K.Igarashi, T.Yamashita, Y.Seyama, M.M.Omata, Y.Kurachi.: "Endothelin-1 and vasopressin activate Ca^<2+>-permeable non-selective cation channels in aortic smooth muscle cells : mechanism of receptor-mediated Ca^<2+>
T.Nakajima、H.Hazama、E.Hamada、S.H.Wu、K.Igarashi、T.Yamashita、Y.Seyama、M.M.Omata、Y.Kurachi.:“内皮素-1 和加压素激活 Ca^<2>-渗透性非渗透性
  • DOI:
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    0
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NAKAJIMA Toshiaki其他文献

NAKAJIMA Toshiaki的其他文献

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{{ truncateString('NAKAJIMA Toshiaki', 18)}}的其他基金

Metagenomics using porous arrowhead devices; from environmental assessment to screening
使用多孔箭头装置的宏基因组学;
  • 批准号:
    23658067
  • 财政年份:
    2011
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Stable-Isotope Probing of plastics film for investigation of surface microbial community
塑料薄膜的稳定同位素探测用于研究表面微生物群落
  • 批准号:
    22350067
  • 财政年份:
    2010
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
The Finding of Factors that Operate on Accounting Standards Development in their Convergence
发现影响会计准则发展趋同的因素
  • 批准号:
    22730375
  • 财政年份:
    2010
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Molecular evolution of the TLR4 gene in the course of primate evolution and their sensitivities to the response to endotoxin
灵长类动物进化过程中TLR4基因的分子进化及其对内毒素反应的敏感性
  • 批准号:
    21590356
  • 财政年份:
    2009
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Identification and its functional analysis of ion channels involving in pathophysiologic remodeling of vascular smooth muscle
血管平滑肌病理生理重塑离子通道的鉴定及其功能分析
  • 批准号:
    20590814
  • 财政年份:
    2008
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Functional analysis for a leader sequence variation Trp16Ser in GnRH which is associated with low bone mineral density among adult women
GnRH 中与成年女性低骨密度相关的前导序列变异 Trp16Ser 的功能分析
  • 批准号:
    18591680
  • 财政年份:
    2006
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Strategies for the identification of susceptibility genes underlying complex diseases through whole-genome linkage disequilibrium (LD) mapping
通过全基因组连锁不平衡(LD)作图鉴定复杂疾病易感基因的策略
  • 批准号:
    14572141
  • 财政年份:
    2002
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Investigation of receptor-activated Ca^<2+>-permeable channels in smooth muscle cells
平滑肌细胞中受体激活的Ca^2-通透性通道的研究
  • 批准号:
    13670691
  • 财政年份:
    2001
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Investigation of ionic channel and its * cance in *
离子通道及其*癌症的研究*
  • 批准号:
    11670568
  • 财政年份:
    1999
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Research of Ionic Channel and Its Intracellular Signalling Pathwa.
离子通道及其细胞内信号通路的研究。
  • 批准号:
    05670413
  • 财政年份:
    1993
  • 资助金额:
    $ 1.28万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

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Daily Regulation of Ionic Currents
离子电流的日常调节
  • 批准号:
    10681356
  • 财政年份:
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  • 资助金额:
    $ 1.28万
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Daily Regulation of Ionic Currents
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Daily Regulation of Ionic Currents
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    8445229
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    8646976
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    8040931
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Daily Regulation of Ionic Currents
离子电流的日常调节
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Daily Regulation of Ionic Currents
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Ionic currents in gastrointestinal smooth muscle
胃肠平滑肌中的离子电流
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    7929151
  • 财政年份:
    2009
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    $ 1.28万
  • 项目类别:
Simultaneous recording of conformational changes and ionic currents of single-molecular ion channels reveals the relationship between membrane potentials and motions of the channels.
同时记录单分子离子通道的构象变化和离子电流揭示了膜电位和通道运动之间的关系。
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