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Function and regulatory mechonismes of ionic chapnels in vasculer snoock muscle cells University of Tokyo

血管斯诺克肌细胞离子通道的功能和调节机制东京大学

基本信息

批准号：
07670760
负责人：
NAKAJIMA Toshiaki
金额：
$ 1.28万
依托单位：
UNIVERSITY of TOKYO
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670760/
关键词：
vascular smooth muscle ionic currents vasopressin esdothelin-1 non-selective cation channel Mg^<2+>fatty a cids Ca^<2+> activated Cl^- current vascular smooth muscle vasopressin non-seledue cation channel Ca^<2+>-activated Cl^- curr

项目摘要

1) To clarify the significance and characterization of Ca^<2+> -permeable nonselective cation channels activated by vasopressin and endothelin-1 (receptor-mediated non-selective cation channels) in vascular smooth muscle cells, the patch clamp techniques, Ca^<2+> or tension measurement were applied in aortic smooth muscle cells. The results suggested that calcium ectry and membrane depolarization elicited by these agonists are partly mediated by a receptormediated Ca^<2+> -permeable non-selective cation channel in vascular smooth muscle cells. Also, we showed the characteristics and activation mechanisms of the channel. Furthermore, we investigated the effects of extracellular Mg^<2+> and omega-3 polyunsaturated fatty acids, which are known to produce vascular relaxation or hypotensive effects, on the receptor-mediated non-selective cation channels. We showed that extracellular Mg^<2+> and omega-3 polyunsaturated fatty acids effectively inhibit receptor-mediated non-selective cation channel. which may play an important role in the regulation of vascular tone.2) In tracheal myocytes, to investigate the activation mechanism and characterization of Ca^<2+> -dependent Cl^- current, the patch clamp technique was applied. We presented that Ca^<2+> -dependent Cl^- current can be activated by SR (sarcoplasmic reticulum) Ca^<2+> release due to neurokinin A or caffeine (through IP_3 or ryanodine receptors) as well as by Ca^<2+> infulx due to the voltage-dependent L-type Ca^<2+> current. It also showed that the functional coupling of ryanodine receptor to the voltage-dependent L-type Ca^<2+> channels exists in tracheal smooth muscle cells.

1)为阐明血管平滑肌细胞中由血管加压素和内皮素1激活的Ca^2+可透过的非选择性阳离子通道（受体介导的非选择性阳离子通道）的意义和特性，应用膜片钳技术、Ca^2+或张力测定在主动脉平滑肌细胞上进行。结果表明，这些激动剂引起的钙离子释放和膜去极化部分是通过血管平滑肌细胞受体介导的Ca^2+可透过的非选择性阳离子通道介导的。同时，我们还对该通道的特性和激活机制进行了初步的探讨。此外，我们研究了细胞外Mg^<2+>和欧米茄-3多不饱和脂肪酸对受体介导的非选择性阳离子通道的影响，已知这些脂肪酸可产生血管松弛或降压作用。我们发现细胞外Mg^2+和ω-3多不饱和脂肪酸有效地抑制受体介导的非选择性阳离子通道。这可能在血管张力的调节中发挥重要作用。2）在气管肌细胞中，应用膜片钳技术研究Ca^<2+>依赖性Cl^-电流的激活机制和特征。我们发现，神经激肽A或咖啡因（通过IP_3或ryanodine受体）引起的肌浆网（SR）Ca^2+释放以及电压依赖性L型Ca^2+电流引起的Ca^2+内流都能激活Ca^2+依赖性Cl^-电流。在气管平滑肌细胞中，Ryanodine受体与电压依赖性L型Ca^<2+>通道存在功能性偶联。