Stable-Isotope Probing of plastics film for investigation of surface microbial community

本研究利用13C对PHB膜进行稳定同位素探测，探索不同土壤样品中PHB的降解作用。从13C标记的PHB膜上提取的DNA进行超离心。得到的DNA被标记为13C。说明膜表面存在的微生物大部分可能是PHB降解菌。通过基于16S rRNA基因的T-RFLP和克隆分析对该13C DNA进行分析，以确定不同的微生物群落。结果表明，PHB膜表面存在不同的微生物群落。

In this study, stable isotope probing of PHB film with 13C in order to explore the PHB degraders from different soil samples. The DNA Extracted from 13C labeled PHB film was subjected to ultracentrifugation. The resultant DNA was found labeled with 13C. It indicates that most of the microbes present on the surface of film might be PHB degraders. This 13C DNA was analyzed through 16S rRNA gene based T-RFLP and clonal analysis to identify different microbial communities. The results indicated the presence of different microbial communities on the surface of PHB films.