Global isolation and classification of Azospirillum species by a new method

通过新方法对固氮螺菌属进行全面分离和分类

• 批准号: 23658080
• 负责人: TANI Akio
• 金额: $ 2.5万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23658080/
• 关键词: 国際情報交換; Azospirllum属細菌; 窒素固定; 根圏; トルコ; Azospirillum属細菌; 国際情報交流

Azospirillum species is azotrophic bacteria symbiotic to plant roots, and capable of promoting plant growth. Isolation of this species is difficult, and their classification and relationship between their host plants is not well elucidated. This study aims to isolate the species as many as possible, to classify them using mass-spectrometry dependent method, and to give a new insight into the classification of the species by finding new species. We found that nitrogen-free medium under micro-aerobic condition is important for their isolation, and we isolated 60 isolates. They were subjected to mass spectrometry for precise classification. We also found some species capable of promoting rice growth by 20%.

固氮螺菌是一种与植物根系共生的固氮细菌，能够促进植物生长。本种的分离是困难的，它们的分类和它们的寄主植物之间的关系还没有很好地阐明。本研究的目的是尽可能多地分离出这些物种，并利用质谱依赖的方法对其进行分类，并通过发现新的物种来对物种的分类提供新的见解。我们发现微氧条件下的无氮培养基对它们的分离很重要，共分离到60个菌株。对它们进行质谱分析以进行精确分类。我们还发现了一些能够促进水稻生长20%的物种。

项目成果

Mangrove sediment, a new source of potential biosurfactant-producing bacteria.

红树林沉积物，潜在的产生生物表面活性剂的细菌的新来源。

• DOI: 10.1007/s13213-012-0424-9
• 发表时间: 2012
• 期刊: Annals of Microbiology
• 影响因子: 3
• 作者: Saimmai;A.;Tani;A.;Sobhon;V.;& Maneerat;S.
• 通讯作者: S.

Extensive reduction in cell viability and enhanced matrix production in Pseudomonas aeruginosa PAO1 flow biofilms treated with D-amino acid mixture

用 D-氨基酸混合物处理的铜绿假单胞菌 PAO1 流生物膜中细胞活力大幅降低，基质生成增强

• 发表时间: 2013
• 期刊: Applied and Environmental Microbiology
• 影响因子: 4.4
• 作者: Zoe Sanchez;Akio Tani;and Kazuhide Kimbara
• 通讯作者: and Kazuhide Kimbara

Dominant Colonization and Inheritance of <i>Methylobacterium</i> sp. Strain OR01 on Perilla Plants

甲基杆菌属的显性定植和遗传。

• DOI: 10.1271/bbb.130207
• 发表时间: 2013
• 期刊: Bioscience, Biotechnology, and Biochemistry
• 作者: Mizuno M;Yurimoto H;Iguchi H;Tani A;Sakai Y
• 通讯作者: Sakai Y

Methylobacterium oxalidis sp. nov., isolated from leaves of Oxalis corniculata.

• DOI: 10.1099/ijs.0.033019-0
• 发表时间: 2012-07
• 期刊: International journal of systematic and evolutionary microbiology
• 影响因子: 2.8
• 作者: A. Tani;N. Sahin;K. Kimbara