Molecular mechanism of site-specificity of Caligus fugu on fins of puffer fish

(1)对寄生桡足类动物的生活史和发育阶段有了新的认识。星后石蕊体的阶段数显然是6个。在某些caligids中暗示了成人的再感染。完整地揭示了一种侵染棘鱼鳍的微小青霉科（pennelid Peniculus minuticaudae）的整个生命周期：孵化阶段为桡足类；没有发现主机切换。鱼鳍很可能被寄生桡足动物的感染阶段感染，但对成年雌性的繁殖力却有限制。(2)生物试验结果表明，河蟹感染桡足类的引诱剂为水溶性引诱剂。通过抑制减法杂交获得了至少10个编码分泌蛋白或酶的基因，该杂交用于分离鳍中表达高于皮肤的基因。这些可能是与感染阶段附着位点特异性相关的候选基因。

(1) The life cycle and developmental stages of some parasitic copepods have been newly revealed by the present investigation. The number of post-naupliar stages of caligids is evidently six. Reinfection of adults is implied in some caligids. The whole life cycle of a pennelid Peniculus minuticaudae infecting fins of filefish is totally disclosed: the hatching stage is copepodids; no host switching is found. It is likely that fish fins are feasibly infected by infective stages of parasitic copepods but so limited for fecundity of adult females. (2) Results of bioassay indicate that attractants for the infective copepodid of Caligus fugu is water-soluble. At least 10 genes coding secretory proteins or enzymes were obtained by suppression subtractive hybridization, which was applied to isolate genes expressed higher in fins compared to skin. These are likely to be candidate genes related to the attachment site-specificity of the infective stage.