Preservation of endangered and rare animalspecies using induced pluripotent stem cells.

使用诱导多能干细胞保护濒危和稀有动物物种。

• 批准号: 23658224
• 负责人: IMAI Hiroshi
• 金额: $ 2.5万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23658224/
• 关键词: 野生動物保護・増殖; 多能性幹細胞

The establishment of induced pluripotent stem (iPS) cells in vole and the characterizations of the established cell lines hasbeen performed as a model experiment for the preservation of endangered and rare animal species. Pluripotent-related four transcription genes were introduced into somatic cells using retroviral vectors. Colonies like as mouse embryonic stem (ES) cells were obtained. These colonies have a part of stem cell potential, but have limited pluripotent characteristics. Further experiments are necessary to establish a system inducing nuclear reprogramming of somatic cells in vole.

田鼠诱导多能干细胞(IPS)的建立及其细胞系的鉴定是保护濒危珍稀动物物种的模式实验。利用逆转录病毒载体将与多潜能相关的四个转录基因导入体细胞。获得了类小鼠胚胎干细胞集落。这些克隆具有部分干细胞潜力，但具有有限的多能性特征。还需要进一步的实验来建立一个诱导田鼠体细胞核重编程的系统。

项目成果

Derivation of murine-ES-cell like iPS cells in canine under the EOS reporter system

EOS报告系统下犬类iPS细胞的鼠类ES细胞的衍生

• 发表时间: 2012
• 作者: Kawaguchi T;Asano R;Tsukiyama T;Minami N;Yamada M;Hatoya S;Sugiura K;Inaba T;Imai H
• 通讯作者: Imai H

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京都大学大学院農学研究科応用生物科学専攻生殖生物学分野

京都大学研究生院农学研究科生殖生物学系、应用生物科学系

Simple and efficient method for generation of induced pluripotent stem cells using piggyBac transposition of doxycyclin-inducible factors and an EOS reporter system

使用强力霉素诱导因子的piggyBac转座和EOS报告系统生成诱导多能干细胞的简单有效的方法

• 发表时间: 2011
• 期刊: Genes Cells
• 影响因子: 2.1
• 作者: Tsukiyama T;Asano R;Kawaguchi T;Kim N;Yamada M;Minami N;Ohinata H and Imai H
• 通讯作者: Ohinata H and Imai H

Derivation of Trophoblast-Like Cells from Bovine Amnion Cells by Piggybac Transposon Vectors Carring Doxycycline-Inducible Reprogramming Factors

通过携带多西环素诱导重编程因子的 Piggybac 转座子载体从牛羊膜细胞中衍生滋养层样细胞

• 发表时间: 2012