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Long term culture and regulation of differentiation of germ cells from the testis in domestic species

Long term culture and regulation of differentiation of germ cells from the testis in domestic species
家养物种睾丸生殖细胞的长期培养和分化调节
批准号：
22380150
负责人：
IMAI Hiroshi
金额：
$12.65万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2010
资助国家：
日本
项目状态：
已结题
起止时间：
2010 至 2012
来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22380150/
关键词：
発生工学生殖幹細胞

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中文摘要
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英文摘要
A long-term culture of male primitive germ cells from bovine testis has been examined. Bovine primitive germ cells can proliferate and survive in culture for over 2 months in the condition with serum-free components and on poly-L-lysine-coated dish. The resulting cell colonies have both pluripotent and stem cell potent characteristics. After culture of cells in the presence of growth factors, cells are differentiated into spermatogonia.
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Determination of distinct germ cell commitment by the specific affinity of the lectin DBA during early development of gonocytes in neonatal mice testis
新生小鼠睾丸性母细胞早期发育过程中凝集素 DBA 的特异性亲和力测定不同生殖细胞的定向
DOI： --
发表时间： 2012
期刊：
影响因子： --
作者： [KimSM, Fujihara M, Goel S, Minami N, Yamada M and Imai H]
通讯作者： Yamada M and Imai H
Identification of reprogramming factors associated with a pluripotent potential of porcine and bovine spermatic stem cells in culture
鉴定与培养的猪和牛精索干细胞的多能潜能相关的重编程因子
DOI： --
发表时间： 2012
期刊： The Proceeding of The 16th Word Congress on In Vitro Fertilization
影响因子： --
作者： [Kim. SM, Fujihara. M, Goel. S, Sahare. M, Minami. N, Yamada. M, Imai. H]
通讯作者： Imai. H
Characterization and in vitro culture of male germ cells from developing bovine testes
发育中牛睾丸雄性生殖细胞的表征和体外培养
DOI： --
发表时间： 2011
期刊： Journal of Reproduction and Development
影响因子： 1.8
作者： [Fujihara M, Kim SM, Minami N, Yamada M and Imai H]
通讯作者： Yamada M and Imai H
Protein kinase A determines timing of early differentiation through epigenetic regulation with G9a
蛋白激酶 A 通过 G9a 的表观遗传调控决定早期分化的时间
DOI： --
发表时间：
期刊： Cell Stem Cell
影响因子： 23.9
作者： [Yamamizu K, Fujihara M, Tachibana M, Katayama S, Takahashi A, Hara E, Imai H, Shinkai Y, Yamashita JK]
通讯作者： Yamashita JK
共 25 条
    Computational Combinatorial Physics by Harmonizing Matroid Theory and Quantum Physics
    • 批准号：
      16K12392
    • 项目类别：
      Grant-in-Aid for Challenging Exploratory Research
    • 资助金额：
      $2.16万
    • 财政年份：
      2016
    • 负责人：
      IMAI Hiroshi
    • 依托单位：
      The University of Tokyo
    Interaction between two motor domains of cytoplasmic dynein stepping along microtubules revealed by cryo-electron microscopy.
    Exploration of synthesis and outward acceleration of circumstellar matter through simultaneous multiple-band high-resolution radio imaging
    • 批准号：
      16H02167
    • 项目类别：
      Grant-in-Aid for Scientific Research (A)
    • 资助金额：
      $29.87万
    • 财政年份：
      2016
    • 负责人：
      IMAI Hiroshi
    • 依托单位：
      Kagoshima University
    Exploiting Matroid Minor Theory and Its Connection with Quantum Computing Models
    • 批准号：
      26540004
    • 项目类别：
      Grant-in-Aid for Challenging Exploratory Research
    • 资助金额：
      $2.33万
    • 财政年份：
      2014
    • 负责人：
      IMAI Hiroshi
    • 依托单位：
      The University of Tokyo
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