Long term culture and regulation of differentiation of germ cells from the testis in domestic species

家养物种睾丸生殖细胞的长期培养和分化调节

• 批准号: 22380150
• 负责人: IMAI Hiroshi
• 金额: $ 12.65万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22380150/
• 关键词: 発生工学; 生殖幹細胞

A long-term culture of male primitive germ cells from bovine testis has been examined. Bovine primitive germ cells can proliferate and survive in culture for over 2 months in the condition with serum-free components and on poly-L-lysine-coated dish. The resulting cell colonies have both pluripotent and stem cell potent characteristics. After culture of cells in the presence of growth factors, cells are differentiated into spermatogonia.

对牛睾丸中的雄性原始生殖细胞进行了长期培养。牛原始生殖细胞在无血清培养条件下和多L赖氨酸涂层培养皿中均能增殖存活2个月以上。由此产生的细胞克隆既具有多能性，又具有干细胞潜能的特征。在含有生长因子的情况下培养细胞后，细胞分化为精原细胞。

项目成果

Determination of distinct germ cell commitment by the specific affinity of the lectin DBA during early development of gonocytes in neonatal mice testis

新生小鼠睾丸性母细胞早期发育过程中凝集素 DBA 的特异性亲和力测定不同生殖细胞的定向

• 发表时间: 2012
• 作者: KimSM;Fujihara M;Goel S;Minami N;Yamada M and Imai H
• 通讯作者: Yamada M and Imai H

Identification of reprogramming factors associated with a pluripotent potential of porcine and bovine spermatic stem cells in culture

鉴定与培养的猪和牛精索干细胞的多能潜能相关的重编程因子

• 发表时间: 2012
• 期刊: The Proceeding of The 16th Word Congress on In Vitro Fertilization
• 作者: Kim. SM;Fujihara. M;Goel. S;Sahare. M;Minami. N;Yamada. M;Imai. H
• 通讯作者: Imai. H

Characterization and in vitro culture of male germ cells from developing bovine testes

发育中牛睾丸雄性生殖细胞的表征和体外培养

• 发表时间: 2011
• 期刊: Journal of Reproduction and Development
• 影响因子: 1.8
• 作者: Fujihara M;Kim SM;Minami N;Yamada M and Imai H
• 通讯作者: Yamada M and Imai H

Protein kinase A determines timing of early differentiation through epigenetic regulation with G9a

蛋白激酶 A 通过 G9a 的表观遗传调控决定早期分化的时间

• 期刊: Cell Stem Cell
• 影响因子: 23.9
• 作者: Yamamizu K;Fujihara M;Tachibana M;Katayama S;Takahashi A;Hara E;Imai H;Shinkai Y;Yamashita JK
• 通讯作者: Yamashita JK

家畜ならびに大型実験動物における多能性幹細胞株の樹立と応用

家畜及大型实验动物多能干细胞系的建立及应用

• 发表时间: 2010
• 作者: Kozue Uchio-Yamada;Kyoko Sawada;Kotaro Tamura;Sumie Katayama;Youko Monobe;Yoshie Yamamoto;Atsuo Ogura;Noboru Manabe;築山智之・今井 裕
• 通讯作者: 築山智之・今井 裕