Stabilization of the population of sea urchins as the materials for physiological and developmental studies

1. 我们尝试在海洋实验室中建立从受精卵中培养海胆幼崽的方法，因为那里的水产养殖设备并不完全可用。3.共获得了7种海胆幼鱼数千只，分别是：大拟中央海胆、粗棘棘海胆、细棘棘海胆、粗圆中央海胆和球状海胆。用30个弃置塑料容器（6个/ml）保存25个经纸过滤的弃置海水。一种硅藻，即毛囊毛藻，被发现是最适合幼虫的食物。当幼虫达到8臂阶段，海胆原基在它们体内长得足够大时，它们被转移到一个500垃圾的塑料容器中，并给予塑料盘子，上面覆盖着硅藻。幼虫在盘子上立即开始蜕变。幼鱼以海草如藻和马尾藻为食。当它们变成5-10毫米时，它们被释放到海洋中。从500只幼崽的容器中获得的最大幼崽数量将是10,000只。

1. We tried to establish the method to rear juvenile sea urchins from fertilized eggs in the marine laboratories where the equipment for aquaculture is not fully available.2. We could obtain several thousands of juvenile sea urchins in 7 species, Pseudocentrotus depressus, Anthocidaris crassispina, Hemicentrotus pulcherrimus, Temnopleurus toreumaticus, Echinostrephus aciculatus, Strongylocentrotus nudus and Mespilia globulus.3. Pluteus lavae were kept in 25 litter paper-filtered sea water in 30 litter plastic vessels (6 plutei/ml). A diatom, Chaetoceros gracilis, was found to be the most suitable food of the larvae.4. When the larvae reached 8-arm stage and the sea urchin primordium grew large enough within them, they were transferred into a 500 litter plastic vessel and given plastic plates which were covered by diatoms. The larvae immediately began metamorphosis on the plates.5. The juveniles were fed by sea weeds such as Ulva and Sargassum. When they became 5-10 mm, they were released into the sea.6. The maximum number of juveniles obtainable from a 500 litter vessel will be 10,000.