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Phylogenetic and evolutionary studies on Prunus species.

李属物种的系统发育和进化研究。

基本信息

批准号：
04454053
负责人：
YOSHIDA Masao
金额：
$ 4.03万
依托单位：
Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04454053/
关键词：
RAPD Phylogenetic studies Classification PCR RFLP Prunus evolution DNA

项目摘要

In order to research relationships of Prunus species, We applied RAPD assay and PCR-RFLP method using three chloroplast DNA domains (psbA,rbcL-ORF106, atpB-rbcL). Matcrial plants of mumc, apricot, plum and peach were offered from Fruit Tree Research Station, and Cherries were offered from Yanmagata Horticultural Research Station. In 1992, We confirmed RAPD assay is suitable for parentage, classification of cultivars and phylogenetic studies in Prunus species using representative cultivars. In 1993, We investigated relationships among many cultivars. Mume cultivars were classified into 6 groups ; "Taiwan-mume", "Koume ", "Chuume", "Oume", "mume-apricot hybrid", "mume-plum hybrid", and it was supposed that Japanese mume is derived from China. Apricot cultivars classified into two groups ; "East Asia", "West Eurasian". Plumcultivars were largely classified into two groups ; "Japanese plum", "European plum". These mumes, apricots and plums are genetically similar and most cultivars have been evoluted with crossing. Peach cultivars are high homologous and were largely classified into two groups ; "P.persica", "P.mira". In 1995, We performed the classification of cherries by RAPD assay, and evolutionary studies of Prunus species by PCR-RFLP method. Prunus species were largely separated into "plum group" and "Cherry group" and Both group were divided in the old time. P.tomentosa, P.japonica which are classified into subgenus Cherry were classified into "Plum group". The experiment of classification of cherries was prevented by earthquake and now, we continue the last part of experiment again.

为了研究李属植物的亲缘关系，本研究采用RAPD和PCR-RFLP方法，对psbA、rbcL-ORF 106、atpB-rbcL三个叶绿体DNA结构域进行了分析。桑树、杏、李、桃等主要植物由果树研究所提供，樱桃由盐县园艺研究所提供。1992年，我们以代表品种为材料，证实RAPD技术适用于李属植物的亲缘关系、品种分类和系统发育研究。1993年，我们调查了许多品种之间的关系。梅品种可分为“台湾梅”、“果梅“、“中华梅“、“青梅”、“梅杏杂种”、“梅梅李杂种”等6大类，推测日本梅是中国的梅品种。杏品种分为两大类;“东亚”，“西欧亚”。李品种主要分为“日本李”和“欧洲李”两大类。这些mumes，numericots和李子是遗传相似的，大多数品种已经通过杂交进化。桃品种间同源性较高，大致可分为两大类：“P.persica”和“P.mira”。1995年，我们利用RAPD技术对樱桃属植物进行了分类，并利用PCR-RFLP技术对李属植物进行了进化研究。李属植物主要分为“李类”和“樱桃类”，这两个类群的划分都是在较早的时期进行的。樱桃亚属的毛樱桃、日本樱桃属于“李组”。樱桃的分级实验因地震而中断，现在，我们再次进行最后一部分的实验。