Approach and penetration of cell membranes by microswimmers and self-propelled particles

微型游泳器和自推进粒子接近和穿透细胞膜

• 批准号: 401729922
• 负责人: Dr. Abdallah Daddi Moussa Ider
• 金额: --
• 依托单位: Abteilung Physik lebender Materie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/401729922?language=en
• 关键词: Approach; penetration; cell; membranes; microswimmers

Our goal in this project is to obtain a full characterization of the approach and penetration of cell membranes by self-propelled active particles both theoretically and numerically. The project is structured into two main parts as outlined below. The understanding of the behavior and dynamics of active particles near membranes, before physical contact takes place, is a crucial ingredient to understand their penetration into the cell interior. The first part of this project will therefore be devoted to the near-membrane dynamics of active particles and microswimmers. For this purpose, we will address analytically, using a continuum description of the fluid, the diffusiophoretic self-propulsion mechanism of a catalytically active particle moving due to a chemical reaction on its surface in the vicinity of a cell membrane. Previous works have only focused on self-propulsion near stiff walls and directed motion near deformable membranes has not been addressed so far. The physical interplay between the chemical activity and membrane elasticity is expected to lead to a very rich and viable phenomenology. Our calculations will be carried out using a variety of different model swimmers including the squirmer model and the higher order representation of the far-field flow. Our theoretical predictions will then be tested and validated by boundary integral simulations. In the second part of the project, we will investigate the penetration process of a self-propelled particle into a cell membrane after physical contact is established. For that aim, we will make use of mesoscopic simulations based on the dissipative particle dynamics method to investigate the physical entry of a self-diffusiophoretic active particle with different sizes and shapes into the lipid bilayer membrane. Additionally, we will explore the fluid- and membrane-mediated interactions between active particles. Our simulations will then be complemented and supplemented by theoretical calculations using asymptotic analysis and scaling arguments in order to examine the wrapping mechanism upon binding of an active article to the cell membrane. Corresponding experiments of biocompatible self-propulsion near and through living cell membranes will be performed by our collaborators.

我们在这个项目中的目标是获得一个完整的表征的方法和渗透细胞膜的自推进活性粒子的理论和数值。该项目分为两个主要部分，概述如下。在物理接触发生之前，对膜附近活性颗粒的行为和动力学的理解是理解它们渗透到细胞内部的关键因素。因此，本项目的第一部分将致力于活性粒子和微泳者的近膜动力学。为此目的，我们将解决分析，使用连续描述的流体，扩散电泳自推进机制的催化活性颗粒移动由于其表面上的化学反应在附近的细胞膜。以前的工作只集中在刚性壁附近的自推进和变形膜附近的定向运动尚未解决到目前为止。化学活性和膜弹性之间的物理相互作用预计将导致非常丰富和可行的现象学。我们的计算将使用各种不同的游泳模型，包括蠕动模型和远场流的高阶表示。我们的理论预测将通过边界积分模拟进行测试和验证。在本项目的第二部分中，我们将研究建立物理接触后自推进粒子进入细胞膜的渗透过程。为此，我们将利用基于耗散粒子动力学方法的介观模拟来研究具有不同尺寸和形状的自扩散泳活性粒子进入脂质双层膜的物理入口。此外，我们将探索活性颗粒之间的流体和膜介导的相互作用。然后，我们的模拟将通过使用渐近分析和缩放参数的理论计算进行补充和补充，以检查活性物质与细胞膜结合后的包裹机制。我们的合作者将在活细胞膜附近和通过活细胞膜进行相应的生物相容性自推进实验。

项目成果

Frequency-dependent higher-order Stokes singularities near a planar elastic boundary: Implications for the hydrodynamics of an active microswimmer near an elastic interface.

平面弹性边界附近频率相关的高阶斯托克斯奇点：对弹性界面附近主动微型游泳器的流体动力学的影响

• DOI: 10.1103/physreve.100.032610
• 发表时间: 2019
• 期刊: Physical review. E
• 作者: A. Daddi-Moussa-Ider;C. Kurzthaler;C. Hoell;A. Zöttl;M. Mirzakhanloo;M. R. Alam;A. M. Menzel;H. öwen;S. Gekle
• 通讯作者: S. Gekle

Hydrodynamics can determine the optimal route for microswimmer navigation

• DOI: 10.1038/s42005-021-00522-6
• 发表时间: 2021-02-02
• 期刊: COMMUNICATIONS PHYSICS
• 影响因子: 5.5
• 作者: Daddi-Moussa-Ider, Abdallah;Lowen, Hartmut;Liebchen, Benno
• 通讯作者: Liebchen, Benno

Towards an analytical description of active microswimmers in clean and in surfactant-covered drops

• DOI: 10.1140/epje/i2020-11980-9
• 发表时间: 2020-09-11
• 期刊: EUROPEAN PHYSICAL JOURNAL E
• 影响因子: 1.8
• 作者: Sprenger, Alexander R.;Shaik, Vaseem A.;Daddi-Moussa-Ider, Abdallah
• 通讯作者: Daddi-Moussa-Ider, Abdallah

State diagram of a three-sphere microswimmer in a channel

• DOI: 10.1088/1361-648x/aac470
• 发表时间: 2018-03
• 期刊: Journal of Physics: Condensed Matter
• 作者: Abdallah Daddi-Moussa-Ider;M. Lisicki;A. Mathijssen;Christian Hoell;Segun Goh;J. Blawzdziewicz;A. Menzel;H. Löwen

Dynamics of a simple model microswimmer in an anisotropic fluid: Implications for alignment behavior and active transport in a nematic liquid crystal

• DOI: 10.1103/physrevfluids.3.094102
• 发表时间: 2018-09-27
• 期刊: PHYSICAL REVIEW FLUIDS
• 影响因子: 2.7
• 作者: Daddi-Moussa-Ider, Abdallah;Menzel, Andreas M.
• 通讯作者: Menzel, Andreas M.