Development and Application of L-band ESR system for in vivo analysis

体内分析L波段ESR系统的开发与应用

• 批准号: 59850126
• 负责人: OGATA Tateaki
• 金额: $ 10.3万
• 依托单位: Yamagata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1984
• 资助国家: 日本
• 起止时间: 1984 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-59850126/
• 关键词: ESR; L-band microwave; loop-gap resonator; on-line digital system; free radical; in vivo; ESRイメージング; L-バンド; ループギャップ型共振器

L-band ESR system which makes it possible to perform in vivo measurements of biological samples has been newly developed in this study.1. Loop-gap resonator having an electric shieldLoop-gap resonator (resonant frequency = 0.7 to 1 GHz) having an electric shield in the loop was made, the characteristics of this resonator were examined by insertion of saline salt solution. The results indicated that electric shield was effective to measurements of wet samples of large quantities.2. On-line system by computerBy connecting computer to L-band ESR spectrometer, scanning of magnetic field, proccessing of ESR signals, and auto control of resonant frequency were carried out. Accumulation and smoothing of signals increased the sensitivity by about 10 times. Auto controller of frequency could afford good ESR spectra without fluctuation of baseline.3. Application to measurements of biological and material samplesBehaviors of exogenous nitroxide radicals in fresh celery and rat could be examined at in vivo state. The change of radical content passing through celery stalk and the decay behavior in the leaves were observed. Attempts of dynamic studies of nitroxide radicals injected in rat were made at the head of rat. Furthermore, in situ measurements of manganese dry cell were performed, and the results suggested that the carbon radical contained in cathode mix was strongly related to the discharge reactions of dry cell.4. ESR imagingIn order to get the distribution of radicals in sample, two-dimensional ESR imaging of various samples was carried out by Zeugmatography.

本研究开发了一种能够对生物样品进行活体测量的L波段ESR系统.制作在环中具有电屏蔽的环隙谐振器（谐振频率= 0.7至1GHz），通过插入盐水溶液来检查该谐振器的特性。结果表明，电屏蔽对大批量湿样品的测量是有效的.计算机在线系统将计算机与L波段ESR谱仪连接，实现了磁场扫描、ESR信号处理和共振频率的自动控制。信号的积累和平滑使灵敏度提高了约10倍。自动频率控制器可提供良好的ESR谱，且基线无波动.应用于生物和材料样品的测量可以在体内状态下检测新鲜芹菜和大鼠体内外源氮氧自由基的行为。观察了芹菜茎中自由基含量的变化及在叶片中的腐烂行为。本文对大鼠头部注射氮氧自由基的动态研究进行了尝试。此外，对锰干电池进行了现场测试，结果表明阴极混合物中的碳自由基与干电池的放电反应密切相关. ESR成像为了得到自由基在样品中的分布情况，我们利用共轭梯度法对不同样品进行了二维ESR成像。

项目成果

第24回ESR討論会講演要旨集. (1985)

第24届ESR研讨会摘要集（1985）。

Chem.Lett.,. 1986-4. (1986)

化学快报，。

Mitsuhiro Ono: "L-band ESR spectrometer using a loop-gap resonator for in vivo analysis" Chem. Lett.491-494 (1986)

Mitsuhiro Ono：“使用环隙谐振器进行体内分析的 L 波段 ESR 光谱仪”Chem。

Mitsuhiro Ono: Chem.Lett.491-494 (1986)

小野光宏：Chem.Lett.491-494 (1986)

第2回日中分析化学合同討論会講演要旨集. (1985)

第二届日中分析化学研讨会演讲摘要集（1985）。