DEVELOPMENT OF FUNCTIONAL DENDORIMER FOR ANALYSIS OF BIORADICAL AND THE APPLICATION

生物自由基分析功能树枝状聚合物的研制及其应用

• 批准号: 09480249
• 负责人: OGATA Tateaki
• 金额: $ 7.62万
• 依托单位: YAMAGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09480249/
• 关键词: DENDRIMER; BIORADICAL; SPIN PROBE; ESR; IN VIVO; RAT; CARBOXY-PROXYL; ニトロキシルラジカル; アスコルビン酸; デンドリマ-; TEMPOL

As one of the in vivo measurement methods for analyzing in vivo reaction of the bioradicals which are related to the physiological and disease phenomena of the living organism, the electron spin resonance (ESR) technique is extremely noticed. However, it is necessary to administer functional spin probe exogenously in order to apply this method to the in vivo measurement.Dendrimer is three-dimensional macromolecule. It is possible to give various functions to the dendrimer, because the introduction of the functional group to the center and the outer shell is easy. And, it is possible to retain the low weight molecular compound in the inside hole, because the inside is sparse on dendrimer with the rise of the generation, and because again, that terminal has the dense structure. In this study, it is a purpose to synthesize dendrimers containing spin probe in the inside and to obtain a basic knowledge on the function of the dendrimers.The dendrimer synthesized in this study included spin probe of about one molecule in the inside, and the water solubility and the organization recognition ability were expected since the end was modified by the sugar chain. Then, the dendrimer which included spin probe (Carboxy-PROXYL) was administered to a rat, and the dynamic behavior was observed on the rat skin. As the result, it was found that the life time of the spin probe was extended on about the double. Since the biocompatibility of the synthetic dendrimer was found to be very low, it was not possible to evaluate the organization recognition ability. The synthesis of dendrimer of the aliphatic polyether was tried in order to obtain the dendrimer of which the biocompatibility is higher. However, it is future problem on the property of the dendrimer.

电子自旋共振（ESR）技术作为分析与生物体生理和疾病现象有关的生物自由基的体内反应的体内测量方法之一，受到了广泛的关注。然而，为了将该方法应用于体内测量，需要外源性给药功能自旋探针。树状大分子是三维大分子。由于将官能团引入到中心和外层很容易，因此可以赋予树状大分子各种功能。并且，可以将低质量的分子化合物保留在内孔中，因为随着代数的增加，内部的树状大分子越来越稀疏，而且，末端的结构也很致密。本研究的目的是合成含有自旋探针的树状大分子，并对树状大分子的功能有一个基本的了解。本研究合成的树状大分子内部包含约1个分子的自旋探针，由于末端被糖链修饰，其水溶性和组织识别能力是预期的。然后，将含有自旋探针（Carboxy-PROXYL）的树状大分子给予大鼠，观察其在大鼠皮肤上的动态行为。结果表明，自旋探针的寿命延长了约两倍。由于合成树状大分子的生物相容性很低，因此无法对其组织识别能力进行评价。为了获得生物相容性较高的树状大分子，对脂肪族聚醚进行了树状大分子的合成。然而，枝状大分子的性质是有待进一步研究的问题。