DEVELOPMENT OF FUNCTIONAL DENDORIMER FOR ANALYSIS OF BIORADICAL AND THE APPLICATION

作为用于分析与活生物体的生理和疾病现象相关的生物自由基的体内反应的体内测量方法之一，电子自旋共振（ESR）技术受到极大关注。然而，为了将该方法应用于活体检测，需要外源性地加入功能性自旋探针。可以赋予树枝状聚合物各种功能，因为官能团容易引入中心和外壳。并且，可以将低分子量化合物保留在内部孔中，因为随着生成的增加，内部在树枝状聚合物上是稀疏的，并且再次因为该末端具有致密结构。本研究的目的是合成内部含有自旋探针的树枝状大分子，并对树枝状大分子的功能有一个基本的认识，本研究合成的树枝状大分子内部含有大约一个分子的自旋探针，由于末端被糖链修饰，因此具有水溶性和组织识别能力。然后，将包含自旋探针（Carboxy-PROXYL）的树枝状聚合物施用给大鼠，并在大鼠皮肤上观察动态行为。结果发现，自旋探针的寿命延长了约一倍。由于发现合成树枝状聚合物的生物相容性非常低，因此无法评价组织识别能力。为了得到生物相容性更好的树枝状大分子，尝试了脂肪族聚醚树枝状大分子的合成。然而，树枝状大分子的性质研究是一个有待解决的问题。

As one of the in vivo measurement methods for analyzing in vivo reaction of the bioradicals which are related to the physiological and disease phenomena of the living organism, the electron spin resonance (ESR) technique is extremely noticed. However, it is necessary to administer functional spin probe exogenously in order to apply this method to the in vivo measurement.Dendrimer is three-dimensional macromolecule. It is possible to give various functions to the dendrimer, because the introduction of the functional group to the center and the outer shell is easy. And, it is possible to retain the low weight molecular compound in the inside hole, because the inside is sparse on dendrimer with the rise of the generation, and because again, that terminal has the dense structure. In this study, it is a purpose to synthesize dendrimers containing spin probe in the inside and to obtain a basic knowledge on the function of the dendrimers.The dendrimer synthesized in this study included spin probe of about one molecule in the inside, and the water solubility and the organization recognition ability were expected since the end was modified by the sugar chain. Then, the dendrimer which included spin probe (Carboxy-PROXYL) was administered to a rat, and the dynamic behavior was observed on the rat skin. As the result, it was found that the life time of the spin probe was extended on about the double. Since the biocompatibility of the synthetic dendrimer was found to be very low, it was not possible to evaluate the organization recognition ability. The synthesis of dendrimer of the aliphatic polyether was tried in order to obtain the dendrimer of which the biocompatibility is higher. However, it is future problem on the property of the dendrimer.