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Establishment of in vitro Propagation System for Selected Melon Lines

甜瓜选系离体繁殖体系的建立

基本信息

批准号：
60860005
负责人：
OHKI Shizuka
金额：
$ 4.99万
依托单位：
Fukui Profectural College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1987
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60860005/
关键词：
Melon pant Breeding Tissue culture 大量増殖

项目摘要

1. For powdery mildew and gummy stem blight resistance selection, the yong plant testing method was esdtablished. However for fusarium wilt, the secection in the highly infected field was suitable.2. We selected 24 lines for netted melon from 1495 plants and 31 lines for open field culture from 1710 plants. Plants with resistance to powdery mildew and with good appearance obtained in high ration, but it was difficult to get plants with gummy stem blight and bearing high sugar content fruits. The selection of the high quality melon plants should be carried out in the second and the third filial generations.3. The in vitro progpagations system was established using nodel tissues as an explant excised from laterla branches. The multiplication rate depended upon cultivers and lines. The secelted line '15-6' was wasiest to propagate. Uisng the media supplamentd by 0.5 to 1.0<micrn>M of BAP for the initial culture and by 0.5<micrn>M of BAP for the subtulture, ws could obtained, by calcuation, (200x6^3)x=3.0x10^5 young plants from one selected plants within 8 months.4. Tissue culture propagated plants was cultured by melon growers. When the soil conditions were poor, the initial gowth was limitedand it resulted smaller lear area and low sugar content of fruits. The diseases resistance was quite higher then the other F1 cultivard testsd. Line '15-6' prodeuced fruits of average 1.4 kg and of average 15.8 ゜Brix. This line was highly estimated by the growars comparing with the other cultivars used there. We are now applying for variety registration naming as 'Bio-Ace'.5. The superiority of this beeding=in vitro nuesery system was proved. We could also apply this system successfully for the bacterial wilt resistant tomato.

1.建立了抗白粉病和胶着病的苗期植株鉴定方法。但对枯萎病而言，以高病田切段为宜。从1495株网纹甜瓜中筛选出24个网纹甜瓜品系，从1710株网纹甜瓜中筛选出31个露地栽培品系。抗白粉病、外观好的植株产量高，但难以获得胶状茎枯病、果实含糖量高的植株。优质甜瓜品种的选育应在子代二、三代进行。建立了以侧枝节状组织为外植体的试管苗增殖体系。增殖率取决于培育者和品系。被割裂的品系‘15-6’繁殖最慢。初始培养用0.5~1.0 mg/L的BAP，继代用0.5~1.0 mg/L的BAP，经计算，8个月内可获得(200×6^3)x=3.0×10~(-5)×10~(-5)×10~(-5)株。组织培养繁殖植株是由甜瓜种植者培养的。土壤条件较差时，果实初期生长受限，叶面积小，含糖量低。抗病性明显高于参试的其他F1品种。品系‘15-6’平均单果重1.4公斤，゜锤度平均15.8。与那里使用的其他品种相比，该品系的种植者高度评价了这一品系。我们正在申请品种注册，命名为‘Bio-Ace’。证明了该体外营养体系的优越性。该系统也可成功应用于番茄抗青枯病的研究。