A simulation study on the removal efficiency of hepatitis B surface antigen in double filtration plasmapheresis

双重过滤血浆置换乙型肝炎表面抗原去除效率的模拟研究

• 批准号: 60870030
• 负责人: ITO Kenichi
• 金额: $ 2.3万
• 依托单位: Kochi Medical School
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60870030/
• 关键词: HBs antigen; Dane particle; chronic hepatitis (type B); double filtration plasmapheresis; Evaflux 4A(Kuraray); Double; filtration; plasma; pheresis

Hepatitis B surface antigen (HBsAg) has been proven to possess antigen-specific and non-specific inhibitory effects to the host-immune system. So we attempt to reduce HBsAg concentration in the serum of the patients with chronic active hepatitis type B to make immunotherapy in hepatitis B virus (HBV) infection more effective. In this paper we reported to removal efficiency of HBsAg in double filtration plasmapheresis (DFP) with Evaflux 4A which showed the selective and stable separation of HBsAg (rejection rate 97%) and DNA-P (almost 100%) compared with albumin (29%) as a secondary filter in simulation model. In the simulation study, HBsAg concentration of pooled plasma was reduced exponentially to 20-30% in 90 min operation. Furthermore with the application of dilution method, 20% increase of HBsAg reduction efficiency or 20% decrease of operation time was achieved. So DFP in order to reduce HBsAg concentration in the blood could be applied for clinical use as a supportive method for immunotherapy of chronic hepatitis B infection.

乙型肝炎表面抗原（HBsAg）已被证明对宿主免疫系统具有抗原特异性和非特异性抑制作用。因此，我们试图降低慢性活动性乙型肝炎患者血清HBsAg浓度，使免疫治疗乙型肝炎病毒（HBV）感染更有效。在本文中，我们报道了Evaflux 4A在双过滤血浆置换（DFP）中对HBsAg的去除效率，与白蛋白（29%）作为二级过滤器相比，仿真模型显示HBsAg（97%）和DNA-P（几乎100%）的选择性和稳定性分离。在模拟研究中，在90分钟的操作中，池血浆中HBsAg浓度呈指数级降低至20-30%。采用稀释法，HBsAg还原效率可提高20%，操作时间可缩短20%。因此，DFP可作为慢性乙型肝炎感染免疫治疗的辅助手段，用于降低血中HBsAg浓度。

项目成果

西原利治,伊藤憲一 他: 肝臓. 26. 696-700 (1985)

Toshiharu Nishihara、Kenichi Ito 等：肝脏。26. 696-700 (1985)

Toshiji, SAIBARA Kenichi, ITO et al.: "The preliminary study on an attempt to remove hepatitis B surface antigen with double filtration plasmapheresis" Japanese Journal of Artificial Organs. 14. 1762-1765 (1985)

Toshiji、SAIBARA Kenichi、ITO 等：“尝试用双重过滤血浆去除术去除乙型肝炎表面抗原的初步研究”日本人工器官杂志。

西原利治,伊藤憲一 他: 人工臓器. 14. 1762-1765 (1985)

Toshiharu Nishihara、Kenichi Ito 等：人工器官。14. 1762-1765 (1985)

Toshiji, SAIBARA Kenichi, ITO et al.: "A simulation study on the removal efficiency of hepatitis B surface antigen in double filtration plasmapheresis" Acta Hepatologica Japonica. 26. 696-700 (1985)

Toshiji、SAIBARA Kenichi、ITO 等：“双重过滤血浆置换中乙型肝炎表面抗原去除效率的模拟研究”Acta Hepatologica Japonica。

Akira, TOMITA Kenichi, ITO et al.: "Inhibition of HBsAg specific cytotoxic T cell activity by excessive HBsAg surface antigen in chronic hepatitis type B" Digestive organ and Immunology. 15. 145-150 (1985)

Akira、TOMITA Kenichi、ITO 等人：“慢性乙型肝炎中过量 HBsAg 表面抗原对 HBsAg 特异性细胞毒性 T 细胞活性的抑制”消化器官和免疫学。