Developmental Studies of Eluorescein Video Angiography and New Analysis Software on Retinal Microcirculation

我们开发了一种测定视网膜平均循环时间(MCT)的系统。荧光素视频血管造影术：将一个超高灵敏度的电视摄像机连接到荧光素摄影单元。眼底荧光素视频抗标记被记录在Bideo磁带中。图像分析和计算：将复制的Bideo血管图传输到图像分析仪，分析视网膜大血管的密度。测量数据回归到表示染料稀释曲线的最佳对数正态分布函数。计算给定视网膜节段的MCT。再现性。进行双重测定。实验证明，该系统作为临床检测视网膜血流的工具具有良好的重复性。正常瓣膜。50只正常眼的MCT为3.00+/-1.26(平均+/标准差)。四个主要视网膜节段之间的视网膜循环时间无明显差异。视网膜分支静脉阻塞(BRVO)：在10例新鲜BRVO中，病变区域的MCT明显长于非病变区域。在22例陈旧性BRVO中，患区MCT短于非患区。糖尿病视网膜病变：对38只糖尿病眼进行MCT测量。单纯性视网膜病变组MCT较正常组短。增殖性视网膜病变的MCT较正常组明显延长。此外，增殖性前期视网膜病变的MCT明显长于增殖性视网膜病变。Buerger病：Berger病患者的MCT延长，尽管存在侧支循环，表明股动脉循环不足。

We developed a system determining retinal mean circulation tiem(MCT).1. Fulorescein video angiography: A superhigh sensitivity TV camera was connected to a fulorescein photograph unit. The fluorescein video antiogram of the ocular fundus was recorded in a bideo tape.2. Image analysis and calculation: The reproduced bideo angiogram was transferred to an image analyzer and densities of large retinal vessels were analyzed. The measured data were regressed to an optimal lognormal distribution function manidesting a dye dilution curve. MCTs of given retinal segements were calculated.3. Reproducibility. Double determination was performed. It was proved that this system had a good reproducibility as a clinical tool measuring retinal circulation.4. Normal valus. MCT in 50 normal eyes was 3.00 +/- 1.26 (mean +/standard deviation). There were no significant differences in retinal circulation time between four major retinal segments.5. Branch Retinal Vein Cclusion (BRVO): In ten fresh BRVO, MCT in the involved area was longer than in the non-involved area. In 22 old BRVO, MCT in the involved area was shorter than in the non-involved area.6. Diabetic Retinopathy: MCT was measured in 38 diabetic eyes. MCT in the simple retinopathy was shorter than in the noermal. MCT in the proliferative retinopathy was longer than in the normal. Furchermore, MCT in the preproliferative retinopathy was longer than in the proliferative retinopathy.7. Buerger disease: MCT of a patient with Berger disease was prolonged in spite of the presence of collateral channels, indicating insufficiency of the retianla circulation.