A Screening of Human Antibodies against Pathogenic Factors from Periodontopathic Bacteria by Enzyme-Linked Immunosobent Assays

1.用免疫印迹法检测牙周病患者血清中抗牙周类杆菌菌毛和膜蛋白的特异性免疫球蛋白G(Ig G)抗体。血清具有强烈的与菌毛反应的倾向，或者确切地说是菌毛的寡聚体结构，具有天然的富含-β结构的构象。然而，血清不与菌毛的一种组成蛋白fimbrlin反应，该蛋白在十二烷基硫酸钠中变性。用免疫印迹法筛选青少年牙周炎患者血清中抗伴生放线嗜血杆菌蛋白的抗体。患者没有出现针对来自几种不同血清学菌株的特殊蛋白成分的特定共同抗体的趋势。

1. A considerable number (more than 50%) of sera from patients with periodontal diseases were shown to have specific immunoglobulin G (Ig G) antibodies against fimbriae and membrane proteins of Bacteroides gingivalis, by using Western blotting analysis. The sera had a strong tendency to react with fimbriae, or exactly oligomeric structures of fimbriae with a native <beta> -structure rich-conformation. However, the sera did not react with fimbrilin, a constituent protein of fimbriae, which is denatured in sodium dodecyl sulfate.2. A screening of antibodies in sera from juvenile periodontitis patients against proteins from Haemophilus actinomycetemcomitans (formerly Actinobacillus) were carried out by using Western blotting analysis. There was no tendency that the patients had specific common antibodies against paticular protein components from several serologically different strains of the organism.