A Screening of Human Antibodies against Pathogenic Factors from Periodontopathic Bacteria by Enzyme-Linked Immunosobent Assays

1.用Western印迹法检测了牙周病患者血清中抗牙龈拟杆菌菌毛和膜蛋白的特异性免疫球蛋白G（IG G）抗体。血清具有与菌毛或具有天然结构富集构象的菌毛的精确寡聚体结构反应的强烈倾向<beta>。然而，血清不与菌毛蛋白反应，菌毛蛋白是菌毛的组成蛋白，其在十二烷基硫酸钠中变性。应用免疫印迹法对青少年牙周炎患者血清中抗伴放线嗜血杆菌蛋白抗体进行了筛选。没有患者对来自几种血清学不同菌株的特定蛋白组分具有特异性共同抗体的趋势。

1. A considerable number (more than 50%) of sera from patients with periodontal diseases were shown to have specific immunoglobulin G (Ig G) antibodies against fimbriae and membrane proteins of Bacteroides gingivalis, by using Western blotting analysis. The sera had a strong tendency to react with fimbriae, or exactly oligomeric structures of fimbriae with a native <beta> -structure rich-conformation. However, the sera did not react with fimbrilin, a constituent protein of fimbriae, which is denatured in sodium dodecyl sulfate.2. A screening of antibodies in sera from juvenile periodontitis patients against proteins from Haemophilus actinomycetemcomitans (formerly Actinobacillus) were carried out by using Western blotting analysis. There was no tendency that the patients had specific common antibodies against paticular protein components from several serologically different strains of the organism.