A Screening of Human Antibodies against Pathogenic Factors from Periodontopathic Bacteria by Enzyme-Linked Immunosobent Assays

酶联免疫分析法筛选抗牙周病细菌致病因子的人类抗体

• 批准号: 60870067
• 负责人: YOSHIMURA Fuminobu
• 金额: $ 2.94万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60870067/
• 关键词: Screening of human antibodies; Antibodies against periodontopathic bacteria; Bacteroides gingivalis; 病原因子; Bacteroides gingivals; Haemophilus actinomycetemcomitans; 歯周病原細菌; Bacteroides gingivalis; 線毛; 抗体; ウエスタンブロット

1. A considerable number (more than 50%) of sera from patients with periodontal diseases were shown to have specific immunoglobulin G (Ig G) antibodies against fimbriae and membrane proteins of Bacteroides gingivalis, by using Western blotting analysis. The sera had a strong tendency to react with fimbriae, or exactly oligomeric structures of fimbriae with a native <beta> -structure rich-conformation. However, the sera did not react with fimbrilin, a constituent protein of fimbriae, which is denatured in sodium dodecyl sulfate.2. A screening of antibodies in sera from juvenile periodontitis patients against proteins from Haemophilus actinomycetemcomitans (formerly Actinobacillus) were carried out by using Western blotting analysis. There was no tendency that the patients had specific common antibodies against paticular protein components from several serologically different strains of the organism.

1. Western blotting分析显示，相当多（超过50%）的牙周病患者血清中含有针对牙龈拟杆菌（Bacteroides gingivalis）菌毛和膜蛋白的特异性免疫球蛋白G （igg）抗体。血清有很强的反应倾向，或确切地说是具有天然< β > -结构丰富构象的低聚结构。然而，血清不与纤原蛋白反应，纤原蛋白是一种组成纤原蛋白，在十二烷基硫酸钠中变性。采用Western blotting方法对青少年牙周炎患者血清中放线菌嗜血杆菌（Haemophilus放线菌）蛋白抗体进行了筛选。没有趋势，病人有特定的共同抗体针对特定的蛋白质成分从几个血清学不同菌株的生物体。

项目成果

Antimicrob.Ag.Chemother. 27-1. (1985)

抗微生物剂.Ag.Chemother。

Yoshimura, F. et al.,: "Fimbriae from the Oral Anaerobe Bacteroides gingivalis : Physical, Chemical and Immunological Properties." J. Bacteriol.163. 730-734 (1985)

Yoshimura, F. 等人：“口腔厌氧菌牙龈拟杆菌的菌毛：物理、化学和免疫学特性。”

Suzuki,Y.et al.: Advances in Dental Research.

Suzuki,Y.et al.：牙科研究进展。

J.Bacteriol.163-2. (1985)

J.Bacteriol.163-2。

Yoshimura,F.et.al.: J.Bacteriol.163. 730-734 (1985)

Yoshimura，F.et.al.：J.Bacteriol.163。