Studies on the functions and genetics of pathogenic factors from periodontopathogens.

牙周病原菌致病因子的功能和遗传学研究。

• 批准号: 61570874
• 负责人: YOSHIMURA Fuminobu
• 金额: $ 1.47万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61570874/
• 关键词: Periodontal disease; Bacteroides gingivalis; Cloning of genes; Anaerobes; Gene for fimbrial subunit; 外膜蛋白質; プロテアーゼ; 付着因子; 嫌気性菌; 線毛遺伝子:付着因子; 歯周病原因子; 病原因子の機能; B.gingvalis; 線毛のクローニング; B.gingivalis

We have carried out the studies on the functions and genetics of pathogenic factors from a periodontopathogen, Bacteroides gingivalis.1, B. gingivalis fimbriae were shown to play an important role on the adherence to buccal epithelial cells.2, In collaboration with Dr. Genco's group, we have cloned the structural gene encoding the fimbrial major subunit, fimbrilin of B. gingivalis. The gene was sequenced and was present as a single copy on the bacterial chromosome, and the codon usage in the gene conformed closely to that expected for an abundant protein.3, The 75-kilodalton major protein, an immunodominant surface antigen was purified to homogeneity from the envelope fraction in B. gingivalis. This protein could be applicable to clinical studies as a specific antigen for an enzyme-linked immunosorbent assay.4, An attempt to clone the gene for a trypsin-like protease in B. gingivalis by screening protease activity expressed remains unsuccessful, suggesting that like fimbrilin, B. gingivalis protease gene may not be expressed in Escherichia coli and pUC plasmids system.

我们对牙周病病原菌牙龈拟杆菌1，B致病因子的功能和遗传学进行了研究。2、与Genco博士的研究小组合作，我们克隆了编码B的菌毛主要亚基fimbrilin的结构基因。牙龈炎该基因被测序，并作为一个单一的拷贝存在于细菌染色体上，在基因中的密码子的使用与预期的丰富的蛋白质。3，75千道尔顿的主要蛋白质，免疫显性表面抗原纯化到同质从包膜部分在B。牙龈炎该蛋白可作为酶联免疫吸附试验的特异性抗原应用于临床研究。4、尝试在B中克隆胰蛋白酶样蛋白酶基因。牙龈炎通过筛选蛋白酶活性表达仍然不成功，表明与伞蛋白、B一样。牙龈蛋白酶基因在大肠杆菌和pUC质粒系统中可能不表达。

项目成果

Dickinson, D. P. et al.: "Molecular cloning and sequence of the gene encoding the fimbrial subunit protein of Bacteroides gingivalis 381." J. Bacteriol.170. 1658-1665 (1988)

Dickinson, D. P. 等人：“编码牙龈拟杆菌 381 菌毛亚基蛋白的基因的分子克隆和序列。”

鈴木武 他: 北海道歯学会雑誌. 8. 15-16 (1987)

Takeshi Suzuki 等人：《北海道牙科学会杂志》。8. 15-16 (1987)

Dickinson,D.P.et al: J.Bacteriol. 170. 1658-1665 (1988)

Dickinson，D.P. 等人：J.Bacteriol。

Suzuki,Y.,et.al: Adv.Dent.Res. 2. 301-303 (1988)

Suzuki,Y.,et.al：Adv.Dent.Res。

Suzuki,Y.,et.al: J.Gen.Microbiol. 134. 2713-2720 (1988)

Suzuki,Y.,et.al: J.Gen.Microbiol。