Prevention, Diagnosis and Treatment of Periodontal Disease Based on Genetic Analysis of Periodontopathogens.
基于牙周病原菌基因分析的牙周病预防、诊断和治疗。
基本信息
- 批准号:02557073
- 负责人:
- 金额:$ 11.33万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Developmental Scientific Research (B)
- 财政年份:1990
- 资助国家:日本
- 起止时间:1990 至 1992
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Virulence factors such as fimbriae, hemagglutinin and an outer membrane protein from porphyromonas (Bacteroides) gingivalis have been studied as to their functions and genes. Downstream and upstream regions from the fimA gene which encodes a major subunit protein (fimbrilin) of fimbriae were cloned in pUC plasmids and a specific expression vector. Proteins with molecular masses of 50- and 80-kilodaltone, encoded by the downstream region were identified by using the bacteriophage T7 RNA polymerase/promoter expression vector system. The two proteins seemed to be associated with fimbriae as minor components based upon the results obtained by Western blot analysis. A hemagglutinin localized in whole envelope of this organism was purified in a homogeneous form. The purified hemagglutinin possessed a thiol protease activity as we have speculated. An N-terminal portion of the gene encoding this bifunctional material was cloned in pUC19. We are trying to clone a C-terminal portion of the gene and to sequence the entire gene in order to estimate the primary structure of this material. The gene encoding a major immunodominant surface protein (the 7 5K protein) of this organism has been cloned. DNA sequencing of the gene is in progress and the work is in the finishing touch.
牙龈卟啉单胞菌(拟杆菌)的毒力因子如菌毛、血凝素和外膜蛋白已被研究其功能和基因。将编码菌毛主要亚基蛋白(fimbrilin)的fimA基因的上游和下游区域克隆到pUC质粒和特异性表达载体中。通过使用噬菌体T7 RNA聚合酶/启动子表达载体系统鉴定了由下游区域编码的分子量为50和80千道尔顿的蛋白质。这两种蛋白质似乎与菌毛作为次要成分的基础上通过Western印迹分析获得的结果。一个血凝素定位于整个包膜的这种生物体被纯化的均匀形式。纯化的血凝素具有巯基蛋白酶活性,正如我们所推测的。将编码该双功能材料的基因的N-末端部分克隆到pUC 19中。我们正试图克隆该基因的C末端部分,并对整个基因进行测序,以估计该物质的一级结构。编码该生物体的主要免疫显性表面蛋白(7 - 5 K蛋白)的基因已被克隆。基因的DNA测序正在进行中,工作已进入收尾阶段。
项目成果
期刊论文数量(21)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
TAKAHASHI,Y.,F.YOSHIMURA,M.KAWANAMI and H.KATO: "Detcotin of Fimbrilin Gene(fimA)in Porphyromonas(Bacteroicres)gingivalis by Sauttern Blot Analysis" J.Periodont.Res. 27. 599-603 (1992)
TAKAHASHI,Y.,F.YOSHIMURA,M.KAWANAMI 和 H.KATO:“通过 Sauttern 印迹分析检测牙龈卟啉单胞菌中 Fimbrilin 基因 (fimA) 的 Detcotin”J.Periodont.Res。
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- 发表时间:
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- 影响因子:0
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- 通讯作者:
Takahashi,Y.,F.Yoshimura,M.Kawanami,and H.Kato: "Detection of Fimbrilin Gene(fimA)in Porphyromonas(Bacteroides)qinqivalis by Southem Blot Analysis" J.Periodont.Res.27. 599-603 (1992)
Takahashi,Y.,F.Yoshimura,M.Kawanami,and H.Kato:“通过 Southem 印迹分析检测卟啉单胞菌(拟杆菌)qinqivalis 中的 Fimbrilin 基因 (fimA)”J.Periodont.Res.27。
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Yoshimura,F.(分担): "分担タイトル:Molecular Biology of Porphyromonas qinqivalis fimbriae Porphyromonas qinqivalis:Biology and Virulence" CRC Press, (1993)
Yoshimura, F.(撰稿人):“撰稿人标题:Qinqivalis 菌毛 Porphyromonas qinqivalis 的分子生物学:生物学和毒力”CRC Press,(1993 年)
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- 影响因子:0
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NISHIKATA,M.and F.YOSHIMURA: "Charatenzation of Porphyromonas(Bacteroides)gingivalis Hemagglutinin as a Protense" Biochem.Biophy.Res Commun.178. 336-342 (1991)
NISHIKATA,M. 和 F.YOSHIMURA:“作为蛋白质的牙龈卟啉单胞菌(拟杆菌)血凝素的特性”Biochem.Biophy.Res Commun.178。
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- 影响因子:0
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Yoshimura, F. and Y. Takahashi: "Cloning of Virulence Factors of Periodontopathic Bacteria" In Periodontal Diseases and Host Immune Responses (S. Hamada, S. C. Holt and J. R. McGhee, eds.). (1991)
Yoshimura, F. 和 Y. Takahashi:“牙周病细菌毒力因子的克隆”在牙周病和宿主免疫反应中(S. Hamada、S. C. Holt 和 J. R. McGhee 编辑)。
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YOSHIMURA Fuminobu其他文献
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{{ truncateString('YOSHIMURA Fuminobu', 18)}}的其他基金
Elucidation of Mechanisms in Fimbrial Morphogenesis in Periodontopathogens
牙周病菌菌毛形态发生机制的阐明
- 批准号:
15591957 - 财政年份:2003
- 资助金额:
$ 11.33万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Isolation and characterization of transposon-derived fimbria-deficient mutants of Porphyromonas gingivalis
牙龈卟啉单胞菌转座子源性菌毛缺陷突变体的分离和表征
- 批准号:
09470398 - 财政年份:1997
- 资助金额:
$ 11.33万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
MOLECULAR BIOLOGICAL STUDIES ON ADHERENT FACTORS OF A PERIODONTOPATHOGEN
牙周病原粘附因子的分子生物学研究
- 批准号:
05454494 - 财政年份:1993
- 资助金额:
$ 11.33万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Cloning of the Gene Encoding a Major Immunodominant Surface Protein of a Peridontopathogen
编码牙周病原菌主要免疫显性表面蛋白的基因的克隆
- 批准号:
02670821 - 财政年份:1990
- 资助金额:
$ 11.33万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
Studies on the functions and genetics of pathogenic factors from periodontopathogens.
牙周病原菌致病因子的功能和遗传学研究。
- 批准号:
61570874 - 财政年份:1986
- 资助金额:
$ 11.33万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
A Screening of Human Antibodies against Pathogenic Factors from Periodontopathic Bacteria by Enzyme-Linked Immunosobent Assays
酶联免疫分析法筛选抗牙周病细菌致病因子的人类抗体
- 批准号:
60870067 - 财政年份:1985
- 资助金额:
$ 11.33万 - 项目类别:
Grant-in-Aid for Developmental Scientific Research
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