Production of antisera against various hormones of exprinmental animals.

为了向基础医学、药学、生物学、畜牧水产等领域的科研人员分发抗血清，我们制备了针对不同实验动物激素的多克隆和单克隆抗血清，以改进免疫方法。在研究期间，制备了抗大鼠催乳素、绵羊催乳素、大鼠生长激素、大鼠促甲状腺激素、大鼠促黄体生成素、促黄体生成素、促性腺激素、α-GT、鸡黄体生成素、牛蛙黄体生成素、bf-FSH、hCG、hCG-lt、β&GT；，脱糖hCG，猪胃动素，人抗紧张素-II，肾素底物，褪黑素，睾酮，雌二醇，皮质醇，皮质酮，雄烯二酮，17-孕酮，孕酮，cAMP，大鼠白蛋白，兔Ig G，豚鼠Ig G，猴Ig G和鼠Ig G，而针对哺乳动物LHRH，bf GTH&lt；α&GT；bf Lh&lt；a&GT；bf FSH&lt；a&GT；绵羊，绵羊FSH，大鼠PRL和霍氏过氧化物酶。从1987年5月起，这些抗血清已初步分发，截至1988年3月底，已向79名科学家供应184瓶抗血清。在抗体制备方面，还对垂体激素的定量提取方法和垂体激素的多态性进行了研究。

With a purpose of distributing antisera to the research scientists in the fields of basic medicine, pharmaceutical sciences, biology, animal husbandry and fishyry, poly clonal and monoclonal antisera were prepared against hormones of various experimental animals with the trials to improve the mothods of immunization. During the research period, polyclonal antisera were produced agains rat PRL, ovine PRL, rat GH, rat TSH, rat LH, oving LH-<beta>, oving TSH-<beta>, ovine GTH-<alpha>, chicken LH, bullfrog LH, bf FSH, hCG, hCG-<beta>, deglycosylated hCG, porcine motilin, human antiotensin-II, renin substrate, melatonin, testosterone, estradiol, cortisol, corticosterone, androstenedione, 17 -progesterone, progesterone, CAMP, rat albumin, rabbit IgG, guinea pig IgG, monkey IgG, and nouse IgG, while monoclonal antibodies were produced against mammalian LHRH, bf GTH-<alpha>, bf LH-<alpha>, bf FSH-<alpha>, ovine LH, ovine FSH, rat PRL, and hoeseradish peroxidase. These antisera have been preliminarily distributed after characterization from May, 1987, and 184 bottles of antisera were supplied to 79 scientists until the end of March, 1988. In connection to the antibody preparation, the quantitative extraction method for pituitary hormoned and polymorphism of pituitary hormones were also studied.