Development of Automatic Instrument for Measurement and Analysis Environmental Mutagens by The Bacillus subtilis Rec-assay.

研制了一种自动检测枯草芽孢杆菌生长的仪器（recc -assay biophotorecoder），在恒温、振荡条件下，用光度计自动测定36根莫诺德试管中的浊度。此外，根据Probit理论和Target理论，可以将生长检测结果引入到rec-assay的分析程序中。自动仪器的运行由一台nec - pc9800个人计算机控制，并由我们开发的软件程序对结果进行分析。因此，一个完整的系统的细菌生长，测量和分析现在是可用的。枯草芽孢杆菌检测是检测环境诱变剂的一种非常有用的工具。最初由Kada博士开发的琼脂平板和条纹法在定量分析方面评估检测结果的应用上存在局限性。然而，液体recc -assay的发展松井使recc -assay更有用的工具。此外，该自动化仪器减少了人工劳动，统计分析结果稳定，可在不同实验室间产生可比较的结果，使rec-assay更容易适用于诱变剂的检测。

An automatic instrument( Rec-assay biophotorecoder) was developed ,which can detect the growth of Bacillus subtilis in 36 Monod tubes by measuring automatically the turbidity with photometers under conditions of constant temperature and acration of shaking. Moreover, the results of detection of the growth can be introduced into a new analysis program of rec-assay based upon Probit theory and Target theory. The operation of the automatic instrument is controlled by a NEC-PC 9800 personal computer and the results are also analyzed by the new soft program developed by us. Thus a complete system of the bacteris growht, measurement and analysis is now available. The Bacillus subtilis rec-assay is a very usuful tool to detect environmental mutagens. The original agar plate and streak method developed by Dr. Kada had a limitation of application to evaluate the assay results in terms of quantitative analysis. However, the development of the liquid rec-assay by Matsui enabled the rec-assay more usuful tool. In addition, this Automatic instrument can make the rec-assay more easy and applicable method to detect mutagens by reducing labour works, obraining stable results with the statistical analysis and producing comparable results among different laboratories.