RISK ASSESSMENT OF ENVIRONMENTAL MICROPOLLUTANTS BY USING DNA STRAND BREAKS IN HUMAN CELLS

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1) DNA strand breaks induced by chlorinated organo compounds containing in tap waterThirteen chlorinated organo compounds were added to the standard of tap water quality in 1993 in Japan. We assessed the DNA strand breaks in human cells induced by eleven these chlorinated chemicals, using the FADU methods. We estimated DNA strand breaks in human fibroblast cells, W138-VA13. DNA strand breaks were induced by 1,2-dichloroethane and 1,3-dichloropropene.2) Detection and Quantification of DNA Strand Breaks in Human Cells Induced by Contaminants in Japanese Tap Waters.We tried to detect DNA strand breaks caused by tap waters. Tap water samples were concentrated using the Sep-pak C_<18> and DNA strand breaks induced in human cells were measured by FADU assay. The tap water samples of Osaka City induced dose dependent increase in DNA strand breaks in human cells. But this DNA toxicity was reduced by rat liver S9 fraction, glutathione and water boiling.