RISK ASSESSMENT OF ENVIRONMENTAL MICROPOLLUTANTS BY USING DNA STRAND BREAKS IN HUMAN CELLS

利用人体细胞 DNA 链断裂评估环境微污染物的风险

• 批准号: 07455211
• 负责人: MATSUI Saburo
• 金额: $ 4.35万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07455211/
• 关键词: HUMANCELLS; DNA STRAND BREAKS; DNA DAMAGE; CHLORINATED ORGANO COMPOUNDS; TAP WATER; DNA鎖切断; ヒト繊維芽細胞; 複合環境汚染物質; FADU法; 色素性乾皮症; repair-assay

1) DNA strand breaks induced by chlorinated organo compounds containing in tap waterThirteen chlorinated organo compounds were added to the standard of tap water quality in 1993 in Japan. We assessed the DNA strand breaks in human cells induced by eleven these chlorinated chemicals, using the FADU methods. We estimated DNA strand breaks in human fibroblast cells, W138-VA13. DNA strand breaks were induced by 1,2-dichloroethane and 1,3-dichloropropene.2) Detection and Quantification of DNA Strand Breaks in Human Cells Induced by Contaminants in Japanese Tap Waters.We tried to detect DNA strand breaks caused by tap waters. Tap water samples were concentrated using the Sep-pak C_<18> and DNA strand breaks induced in human cells were measured by FADU assay. The tap water samples of Osaka City induced dose dependent increase in DNA strand breaks in human cells. But this DNA toxicity was reduced by rat liver S9 fraction, glutathione and water boiling.

1)自来水中含氯有机物引起的DNA链断裂日本1993年在自来水水质标准中增加了13种含氯有机物。我们使用FADU方法评估了11种氯化化合物诱导的人类细胞DNA链断裂。我们估计了人成纤维细胞W138-VA13的DNA链断裂。用1，2-二氯乙烷和1，3-二氯丙烯诱导DNA链断裂。2)日本自来水污染物致人细胞DNA链断裂的检测与定量。用Sep-pak C_&lt；18&gt；浓缩自来水样品，用FADU法检测细胞DNA断裂。大阪市自来水样品致人细胞DNA链断裂呈剂量依赖性增加。但这种DNA毒性可通过大鼠肝脏S9组分、谷胱甘肽和水煮来降低。

项目成果

松井三郎: "Molecular analysis of mutations induced by 2-chloroacetaldehyde, the ultimate Caranogenic form of vinyl chloride, in human cells using shuttle vectors" Carcinogenesis. 16. 2389-2394 (1995)

Saburo Matsui：“使用穿梭载体对人类细胞中由 2-氯乙醛（氯乙烯的最终致癌形式）诱导的突变进行分子分析”致癌作用。

T.Matsuda: "Detection and quantification of DNA strand breaks in human cells induced by contaminants in Japanese tap waters" Wat.Sci.Tech. 33-6. 297-304 (1996)

T.Matsuda：“日本自来水污染物引起的人体细胞 DNA 链断裂的检测和定量”Wat.Sci.Tech。

T.Matsuda: "DNA strand breaks in human cells induced by chlorinated organo compounds containing in tap water" Environ.Mut.Res.Commun. 17. 161-167 (1995)

T.Matsuda：“自来水中含有的氯化有机化合物诱导人体细胞中的 DNA 链断裂”Environ.Mut.Res.Commun。

松井三郎: "Detection and Quantification of DNA strand Breaks in Human cells Induced by Contaminants in Japanese Tap waters." Water Science and Technology. 33. 未定 (1996)

Saburo Matsui：“日本自来水污染物引起的人体细胞 DNA 链断裂的检测和定量。”33。待定（1996 年）。

T. Matsuda: "Detection and quantification of DNA strand breaks in human cells induced by contaminants in Japanese tap waters" Wat. Sci. Tech.13 (6). 297-304 (1996)

T. Matsuda：“日本自来水污染物引起的人体细胞 DNA 链断裂的检测和定量”Wat。