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Novel Characterization of Humic Substarces and this Role in the Behavior of Environmental Toxic Substances using HPLc/MS/MS

使用 HPLc/MS/MS 对腐殖质物质进行新表征及其在环境有毒物质行为中的作用

基本信息

批准号：
10305039
负责人：
MATSUI Saburo
金额：
$ 15.81万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

1. Isolation and Characterization of NOM from Lake Biwa JapanLake Biwa natural organic matter (NOM) was isolated and fractionated from the Southern and Northern Lakes using the sequential membrane system installed at Kyoto University. TOC analysis of the fractionated samples indicated that the high molecular weight fractions (> 1,000 Da) of both lakes were less than 2O%, which is much less than the values reported for Lake Michigan and Suwanee River NOM (76 and 37%, respectively). The SUVA (Specific Ultra Violet Absorbance) was also measured for the fractionated Lake Biwa NOM.The higher molecular weight fractions had smaller SUVA values. Since SUVA corresponds to the content of aromatic compounds, the results indicate that the aromatic compounds in Lake Biwa NOM are less in the higher molecular weight fractions.2. Sorption of Micro-organic Pollutants with Lake Biwa NOM.JapanPyrene was selected from polynuclear aromatic hydrocarbons (PAH) as a micro-organic pollutant. The sorption onto … More the fractionated Lake Biwa NOM was measured by batch sorption experiments. The experimental results indicated that the sorption of pyrene was controlled by the molecular weight (i.e., the greater sorption was observed for the higher molecular weight fractions of Lake Biwa NOM) not by SUVA (i.e., no distinct relationship was observed between the sorption and SUVA).3. Development of an Alternative to K_<ow> as a Predictor of Contaminant ToxicityThe aqueous-phase sorption of micro-organic pollutants into liposome and the effects of coexisting humic substances on the sorption were experimentally evaluated. The liposome, used for simulating living cell membrane, was synthesized in the laboratory, and two PAHs (pyrene and phenanthrene) were used for the experiment. The batch sorption results indicate that the sorption into liposome could be an alternative to K_<ow> for estimating the transport of micro-organic pollutants into living cells and humic substances retarded the transport by binding with micro-organic pollutants in the aqueous phase. Less

1.日本琵琶湖天然有机物（NOM）的分离和表征利用京都大学安装的连续膜系统从南部和北方湖泊中分离和分级琵琶湖天然有机物（NOM）。分馏样品的TOC分析表明，两个湖泊的高分子量部分（> 1，000 Da）均小于20%，远低于密歇根湖和Suwanee河NOM报告的值（分别为76%和37%）。还测量了分馏的琵琶湖NOM的苏瓦（比紫外线吸光度）。分子量较高的馏分具有较小的苏瓦值。由于苏瓦与芳香族化合物的含量相对应，因此结果表明，琵琶湖NOM中芳香族化合物在高分子量组分中含量较少.日本琵琶湖对微量有机污染物的吸附研究从多环芳烃中选择芘作为微量有机污染物。吸附到 ...更多信息分级的琵琶湖NOM通过分批吸附实验测量。实验结果表明，芘的吸附受分子量控制（即，对于较高分子量的琵琶湖NOM级分观察到更大的吸附）而不是通过苏瓦（即，吸附量与苏瓦无明显相关性.开发一种替代K_2<ow>的污染物毒性预测因子实验研究了脂质体对水中微量有机污染物的吸附以及共存腐殖物质对吸附的影响。在实验室合成了模拟活细胞膜的脂质体，并以芘和菲两种多环芳烃为实验对象。实验结果表明，脂质体吸附法可以代替K_1法<ow>估算微生物污染物向活细胞的迁移，腐殖物质通过与水相中的微生物污染物结合而阻碍了微生物污染物的迁移。少